Hitachi, Ltd.

Kakuro Hirai

Manabu Shiozawa

Takao Watanabe

Masaharu Kiyama

Erino Matsumoto

Provided is a novel cell sheet evaluation method. A cell sheet evaluation method includes: a step of analyzing, based on an observed image of a cell sheet, a characteristic indicating shape uniformity of cells constituting the cell sheet; and a step of evaluating a binding state between cells in the cell sheet based on the analysis result.

Cell sheet evaluation method

In multicolor Coherent anti-Stokes Raman Scattering, a fingerprint region is a region densely filled with signals, and information about an intensity or spatial distribution of the signals is important for cell analysis. However, when laser power was increased due to the low signal intensity, there was a problem that cells were damaged. Thus, the present invention was configured such that the number of pulses of laser light emitted from a short pulse laser light source was changed by a pulse modulator. Accordingly, damage to the cells can be suppressed without significant reduction in signal intensity.

Optical measurement apparatus and optical measurement method

Exemplary embodiments of the present disclosure relate to providing an optical apparatus that is capable of acquiring representative Raman spec trams with high reliability from hyper spectrum images of samples. An optical apparatus according to this disclosure: receives a specifying input that specifies a specific Raman spectrum bandwidth as a reference signal; and extracts a Raman spectrum for a measurement point where an intensity of the reference signal is at or above a reference intensity.

Optical apparatus and optical measurement method

The invention is directed to a light measuring apparatus which is configured to generate broadband light from a laser beam emitted from a light source, the broadband light having a broader band than that of the laser beam, to divide the broadband light into a long wavelength component and a short wavelength component, to perform CARS measurement using the long wavelength component, and to perform fluorescence observation using the short wavelength component.

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Tokyo, Japan

Kakuro Hirai