Macquarie University

Kaisu Milja Helena Nevalainen

Moreland David Gibbs

Peter Leonard Bergquist

A method for gene shuffling to form a mutant or chimeric gene, the method comprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5' and 3&#x2032; degenerate ends.

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North Epping, Australia

Kaisu Milja Helena Nevalainen