Leica Microsystems Cms Gmbh

Kai Ritschel

Christian Schumann

Benjamin Deissler

Mate Beljan

Volker Schacht

Oliver Keul

Patric Pelzer

Wernher Fouquet

Jose Miguel Serra Lleti

Marco Bingel

Oliver Schlicker

Gheorghe Cojoc

Dennis Jaehnert

Dennis Jähnert

A fluorescence microscope system for imaging a sample having at least two different fluorophores includes an illumination system configured to emit illumination light for exciting the fluorophores; an optical detection system configured to generate images of the sample based on fluorescence light emitted by the excited fluorophores; and a control unit configured to determine whether to image the sample in a concurrent imaging mode or in a sequential imaging mode, based on at least one characteristic of each of the fluorophores and based on at least one parameter of the optical detection system and/or the illumination system. In the concurrent imaging mode, the fluorophores are imaged simultaneously, and in the sequential imaging mode, the fluorophores are divided into a first group and at least one second group, and fluorophores of the first group and the at least one second group are imaged subsequently.

Fluorescence microscope system and method

Embodiments of the present invention relate to an optical imaging system, and to methods, systems, and computer programs for such an optical imaging system. The methods comprise obtaining first image data of an imaging device of the imaging system, the first image data comprising a representation of a pattern. The methods comprise obtaining second image data of the pattern from the imaging device after the pattern has been displaced by a stage of the optical imaging system by a distance in a dimension defined relative to the stage. The methods comprise determining an offset between the patterns of the first and second image data in two dimensions. The methods comprise calculating a conversion parameter based on the offset and the distance. A first method comprises controlling a drive unit configured to displace the stage based on the conversion parameter.

Optical imaging system, methods, systems, and computer programs

A control device for a microscope includes an actuator configured to shift a microscopic field of view relative to a sample, and an operating device configured to be operated by a user to control the actuator in accordance with a response characteristic determining a shift sensitivity. The field of view is shifted relative to the sample in response to a user operation of the operating device. The control device further includes a processor configured to determine a total visual magnification, and to control the response characteristic of the operating device based on the total visual magnification. The field of view is visualized by the microscope to the user based on the total visual magnification.

Control device for a microscope

A method of analyzing a mixed fluorescence response of a plurality of fluorophores in a microscopic sample includes reconstructing individual fluorescence responses from a mixed fluorescence response using spectral un-mixing based on reference emission spectra for fluorophores to be reconstructed, and a procedure for determining and validating reference emission spectra including providing a plurality of image acquisition settings for a sequence of images of the sample equal to, or greater than, the plurality of fluorophores and including an illumination setting for each image, acquiring the sequence of images using the plurality of image acquisition settings and storing each image together with the corresponding illumination setting, determining candidate reference emission spectra for the fluorophores to be reconstructed from the sequence of images of the sample using one or more reference emission spectra determination algorithms, and conditionally using the candidate reference emission spectra as the reference emission spectra in the spectral un-mixing.

Method of analyzing a mixed fluorescence response of a plurality of fluorophores, fluorescence analyzer, fluorescence microscope and computer program

A method for automatically ascertaining illumination brightnesses to be adjusted of at least two light sources for exciting at least one respective fluorophore in a sample to be imaged in a fluorescence microscope includes separately controlling, in terms of illumination brightness, each of the at least two light sources, detecting an image intensity of a microscopically imaged sample with at least two detectors, and automatically ascertaining the illumination brightnesses to be adjusted of the at least two light sources in such a way that a predefined setpoint of a signal-to-noise ratio is reached for each fluorophore. In order to ascertain the illumination brightnesses of the at least two light sources, cross-talk of a detector for different emission spectra of the fluorophores and/or cross-excitation of a fluorophore for different illumination spectra of the light sources are/is taken into account.

Method for adjusting the illumination in a fluorescence microscope, and corresponding fluorescence microscope

A multispectral microscope system includes a first detector element for capturing a first image of a sample in a first spectral channel, and at least a second detector element for capturing a second image of the sample in a second spectral channel. The first detector element includes a first detector array. The second detector element includes a second detector array different from the first detector array. The microscope system further includes a processor for determining a spatial correlation between the first and second images based on a spectral crosstalk between the first and second spectral channels and registering the first and second images based on the spatial correlation.

Multispectral microscope system and method for registering first and second images by means thereof

A control device for a microscope includes an operating device, an actuator and a processor. The operating device is configured to be operated by a user to vary focusing and/or positioning of an optical imaging system of the microscope relative to a sample. The actuator is configured to adjust an aperture of a detection pinhole which is included in the microscope so as to eliminate out-of-focus light from detection light which is directed by the optical imaging system onto a detector of the microscope. The processor is configured to detect a predetermined operating condition in response to a user operation of the operating device and to control the actuator to vary the aperture of the detection pinhole upon detection of the predetermined operating condition.

An optical imaging device for a microscope comprises a first optical system configured to form a first optical image corresponding to a first region of a sample in accordance with a first imaging mode, a second optical system configured to form a second optical image corresponding to a second region of said sample, wherein said first and second regions spatially coincide in a target region of said sample and said first and second imaging modes are different from each other, a memory storing first distortion correction data suitable for correcting a first optical distortion caused by said first optical system in said first optical image, second distortion correction data suitable for correcting a second optical distortion caused by said second optical system in said second optical image, and transformation data suitable for correcting positional misalignment between said first and second optical images, and a processor which is configured to process first image data representing said first optical image based on said first distortion correction data for generating first distortion corrected image data, to process second image data representing said second optical image based on said second distortion correction data for generating second distortion corrected image data; and to combine said first and second distortion corrected image data based on said transformation data for generating combined image data representing a combined image which corresponds to said target region of said object.

Optical imaging device for a microscope

A microscope includes a digital imaging device. The digital imaging device comprises a processor which is configured to: obtain a number of digital grayscale images of an object at a number of different spectral sensitivities, each of the digital grayscale images including grayscale information based on a different one of the different spectral sensitivities, obtain a number of weighting factors for each of the digital grayscale images, the weighting factors being allocated to a number of color channels defining a predetermined color space, and synthesize a digital color image of the object from the digital grayscale images in the color space by distributing the grayscale information of each grayscale image over the color channels in accordance with the weighting factors.

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Heuchelheim, Germany

Kai Ritschel