University of Kentucky Research Foundation

Chinese Academy of Fishery Sciences

Kai Liu

Daniel L Lau

Laurence G Hassebrook

Gangchun Xu

Hongxia Li

Pao Xu

Yifan Tao

Haojun Zhu

Jie He

Jun Qiang

Yongchang Wang

Zheming Cao

The present disclosure provides a combination of antisense RNA sequences and use thereof in the production of tilapia with degenerated sexual organs, belonging to the technical field of molecular biology and reproductive biology, the combination of antisense RNA sequences includes antisense RNA of steroidogenic factors SF1-1 and SF1-2; the nucleotide sequences of Anti-SF1-1-I, Anti-SF1-1-II, Anti-SF1-2-I and Anti-SF1-2-II are set forth in SEQ ID NO:1-SEQ ID NO:4 respectively. The method of the present disclosure introduces antisense RNA fragments into the eggs through the fertilization hole to realize effective and accurate targeted intervention for regulating the gene expression, and the method has the advantages of simple operation, minimal egg damage, high success rate, stable phenotype after breeding, and excellent application prospects.

Combination of antisense RNA sequences and use in the production of tilapia with degenerated sexual organs

A computer-implemented process, system, and computer-readable storage medium having stored thereon, program code and instructions for 3-D triangulation-based image acquisition of a contoured surface/object-of-interest under observation by at least one camera, by projecting onto the surface-of-interest a multi-frequency pattern comprising a plurality of pixels representing at least a first and second superimposed sinusoid projected simultaneously, each of the sinusoids represented by the pixels having a unique temporal frequency and each of the pixels projected to satisfy where Iis the intensity of a pixel in the projector for nprojected image in a particular instant in time; K is an integer representing the number of component sinusoids (e.g., K=2 for a dual-frequency sinusoid pattern, K=3 for a triple-frequency sinusoid, each component sinusoid having a distinct temporal frequency, where K is &#x2266;(N+1)/2.

Dual-frequency phase multiplexing (DFPM) and period coded phase measuring (PCPM) pattern strategies in 3-D structured light systems, and lookup table (LUT) based data processing

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Wuxi, China

Kai Liu