Valtion Teknillinen Tutkimuskeskus

Teknologian Tutkimuskeskus Vtt

Kai Kolari

Ari Hokkanen

Hans Söderlund

Kari Kataja

Ingmar Stuns

Reetta Satokari

Päivi Heimala

Stella Rovio

Jan Rautio

Juha-Pekka Pitkänen

Jari Kauhaniemi

Pekka Savolahti

Jaana Uusitalo

A microfluidic chip device (MCD) and its use for performing miniaturized assays on magnetic microbeads (MMs) are described. The MCD is particularly useful for carrying out miniaturized transcript analysis by aiding affinity capturing (TRAC) assays, including PCR. The MCD comprises at least one reaction chamber with sealable liquid connections and at least one fluidic pillar filter in each chamber. The fluidic pillar filter comprises rods with spacings allowing MMs to pass. The sealable liquid connections feed liquid to the reaction chamber, wherein air bubbles are removed. The liquid stream contacts the MMs, which are manipulated with a magnetic rod. The liquid connections enable trapping of the MMs behind the pillar filters or in the channel, while the liquid is changed.

Microfluidic chip devices and their use

A sample port includes a body equipped with an internal cavity and two plungers arranged moveably in the internal cavity, whereby the plungers can be pressed against each other in the internal cavity, in order to compress a sample. At least one of the plungers can be moved into a reactor in order to collect a sample. The sample port also includes a sample chamber, which is formed by a space remaining between the internal cavity and the plungers. At least one sample-container connection is connected in connection with the internal cavity in order to collect the sample from sample chamber. In addition, the sample port has a filter which is provided to at least either plunger for separating a liquid component of the sample from a solid component and means for leading the liquid component of the sample out of the sample chamber.

Sample port, multi-layer filter, sampling method, and use of a sample port in sampling

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Espoo, Finland

Kai Kolari