University of North Dakota

K C Sekhar

Masaru Miyagi

Optimized enzymatic conditions incorporate a single oxygen atom into digested peptides using a peptidase. The incorporation of a single oxygen atom is especially useful for proteolyticO labeling in comparative proteomics. The optimized proteolyticO labeling minimizes the generation of a mixture of isotopic isoforms of the peptides resulting from incorporation of either one or twoO atoms. The outcome is accurate quantification of isotopically labeled peptides.

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Grand Forks, ND, United States of America

K C Sekhar