Prolexys Pharmaceuticals, Inc.

Glycomira Therapeutics, Inc.

Justin Savage

Glenn D Prestwich

Thomas P Kennedy

Moritz Von Rechenberg

John Jay Boniface

Paul B Robbins

Won Yong Lee

John M Peltier

Vladimir Kery

Abigail Pulsipher

Described herein are methods for preventing a serious health consequence and/or tissue damage in a subject after the subject has been exposed to ionizing radiation and/or chemotherapy. The methods involve administering to the subject a sulfated polysaccharide or the pharmaceutically acceptable salt or ester thereof after the subject has been exposed to ionizing radiation and/or chemotherapy. The methods described herein are most beneficial to cancer patients who suffer from mucositis after exposure to ionizing radiation.

Methods for preventing a serious health consequence and/or tissue damage after exposure to ionizing radiation and/or chemotherapy

The present invention provides a method for purifying a protein complex and the components comprising the complex from a cell, a cell or tissue lysate or a whole organism by employing a combined set of affinity tags of high affinity, specificity and ease of elution. The method involves using a protein or a peptide modified to contain one or more affinity tags separated by one or more specific protease cleavage sites to isolate any interacting proteins or fragments thereof. Specifically exemplified is a method employing a modified bait containing AviTag or GST and a removal tag of Hishexapeptide positioned proximal to the bait such that any excess bait can be efficiently removed from the purified complex yielding enriched interacting proteins prior to subjecting the complex for further identification of individual components. The identification of the proteins or fragments thereof contained in the protein complex will provide new targets for the identification of new pharmaceuticals and diagnostic tools.

Method for purification of a protein complex and identification of its components

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North Salt Lake, UT, United States of America

Justin Savage