Src Corporation

Justin P Andrews

Timothy Francis Moshier

Kenton Arthur Doctor

Stephen R Houghton

Stacey Ann Massulik

Jeffrey Harold Mills

Huda Sirageldin Suliman

Frances Louise Stites

Lisa Helen Chamberlin

A kit and method for detection of a target in a sample. An assay mixture provided in the kit and used in the method includes a first probe with a first antibody recognizing a first epitope of the target and conjugated to an RNA oligonucleotide; a second probe with a second antibody recognizing a second epitope of the target and conjugated to a DNA oligonucleotide; a reverse primer with a first region complimentary to the RNA oligonucleotide and a second region complimentary to the DNA oligonucleotide; and a reverse transcriptase that creates a DNA transcription product from the RNA oligonucleotide using the reverse primer only if the RNA oligonucleotide and the DNA oligonucleotide are in close proximity. If the target is present in the sample, the reverse primer binds the RNA oligonucleotide and the DNA oligonucleotide to bring the RNA oligonucleotide and the DNA oligonucleotide in close proximity.

Detecting a target molecule in a sample using a dual-antibody quantitative fluorescence-based detection method

Growing community of inventors

Camillus, NY, United States of America

Justin P Andrews