Uea Enterprises Limited

Progenika Biopharma S.a.

Justin Joseph O'Grady

Gemma Louise Kay

John Richard Wain

Solomon Mwaigwisya

Antonio Martinez

Lourdes Palacios

Nerea Bartolome

Alp Aydin

Themoula Charalampous

Riccardo Scotti

Provided is a method for depleting host nucleic acid in a biological sample, said sample having been previously obtained from an animal host, said method comprising the steps of (a) adding a cytolysin, or an active variant thereof, to said sample; and (b) carrying-out a process to physically deplete nucleic acid released from host cells within said sample or otherwise render such nucleic acid unidentifiable.

Method for nucleic acid depletion

The present invention provides a method for depleting host nucleic acid in a biological sample, said sample having been previously obtained from an animal host and having been subjected to treatment to lyse host cells present in the sample, and having been subjected to a treatment to deplete nucleic acid released from host cells within said sample or otherwise to render such nucleic acid unidentifiable, the method comprising: (a) depleting host mitochondria or host mitochondrial DNA (mtDNA) present in the treated sample; and (b) extracting and/or analysing remaining nucleic acid from the treated sample. Also provided is a related kit comprising reagents for performing the method. Further claimed is a method for isolating mitochondrial DNA after host cells have been lysed and host cell nucleic acids have been removed from the sample.

Mitochondrial nucleic acid depletion and detection

Provided are methods, compositions and kits for depleting host nucleic acid in a biological sample, said sample having been previously obtained from an animal host.

Method for digesting nucleic acid in a sample

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Norwich, United Kingdom

Justin Joseph O'Grady