Illumina Corporation

Illumina Cambridge Limited

Justin Fullerton

M Shane Bowen

Jeffrey S Fisher

Lewis J Kraft

Boyan Boyanov

Andrew Antony Brown

Wayne N George

Maria Candelaria Rogert Bacigalupo

Brian Douglas Mather

Sang Ryul Park

Ludovic Vincent

Sahngki Hong

Tarun Kumar Khurana

Yir-Shyuan Wu

Arnaud Rival

Xi-Jun Chen

Hui Han

Mathieu Lessard-Viger

Yasaman Farshchi

Li Liu

Min-Jui Richard Shen

Dajun Yuan

Shannon Kaplan

Bala Murali Venkatesan

Sean M Ramirez

Rigo Pantoja

Kathryn M Stephens

Bala Murali K Venkatesan

Alex So

John Gerhardt Earney

Steven Modiano

Timothy J Wilson

Anne Jager

Neeraj Salathia

Sang Park

Kaleb Smith

Timothy Wilson

An example of a flow cell includes a substrate, a plurality of chambers defined on or in the substrate, and a plurality of depressions defined in the substrate and within a perimeter of each of the plurality of chambers. The depressions are separated by interstitial regions. Primers are attached within each of the plurality of depressions, and a capture site is located within each of the plurality of chambers.

Flow cells

An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

Flow cells using sequencing-ready nucleic acid fragments attached to carrier beads immobilized at capture sites of a plurality of chambers

Flow cells with chambers, depressions, and capture sites

Presented are methods and compositions for spatial detection and analysis of nucleic acids in a tissue sample. The methods can enable the characterization of transcriptomes and/or genomic variations in tissues while preserving spatial information about the tissue.

Spatial mapping of nucleic acid sequence information

An example method includes providing a working stack having a first substrate layer, a second substrate layer, and a radiation-absorbing material disposed between the first and second substrate layers. The working stack includes a cavity therein having a designated liquid. A bonding interface is defined between the radiation-absorbing material and at least one of the first substrate layer or the second substrate layer. The bonding interface has a film of the designated liquid. The method also includes directing radiation onto the bonding interface to form a perimeter seal. The perimeter seal separates the cavity from an outer area of the bonding interface. The method also includes directing the radiation onto the outer area of the bonding interface to secure the first and second substrate layers together. The perimeter seal impedes an ingress of bubbles from the outer area into the cavity as the radiation is directed onto the outer area.

Fluidic devices and methods of manufacturing the same

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San Diego, CA, United States of America

Justin Fullerton