Purdue Research Foundation

Justin Allen Newman

Garth Jason Simpson

Charles Addison Bouman

Ryan Douglas Muir

Shane Zachary Sullivan

Mark Carlsen

Suhas Sreehari

Nicholas Roman Pogranichniy

Nicole Mulready Scarborough

A beam-scanning optical design is described for achieving up to kHz frame-rate optical imaging on multiple simultaneous data acquisition channels. In one embodiment, two fast-scan resonant mirrors direct the optical beam on a circuitous trajectory through the field of view, with the trajectory repeat-time given by the least common multiplier of the mirror periods. Dicing the raw time-domain data into sub-trajectories combined with model-based image reconstruction (MBIR) 3D in-painting algorithms allows for effective frame-rates much higher than the repeat time of the Lissajous trajectory. Because sub-trajectory and full-trajectory imaging are different methods of analyzing the same data, both high-frame rate images with relatively low resolution and low frame rate images with high resolution are simultaneously acquired.

High frame-rate multichannel beam-scanning microscopy

Methods for determining the 3-D structures of proteins. Such a method includes incorporating a compound into a protein crystal such that the compound enhances the activity of the protein crystal to second harmonic generation, illuminating the protein crystal with a sufficiently intense light to cause second harmonic generation by the protein crystal, and detecting a second harmonic generation response produced by the protein crystal that is suitable for protein structure determination by diffraction analysis.

Methods for second harmonic generation imaging of protein crystals

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West Lafayette, IN, United States of America

Justin Allen Newman