Abacus Diagnostica Oy

Other

Jussi Nurmi

Teemu Korpimäki

Timo Lovgren

Timo Lövgren

Hannu Kojola

Anniina Syrjälä

Piia Von Lode

Virve Hagren

Antti Iitia

Antti Iitiä

A method for detecting the presence of a target polynucleotide in a sample, including providing a mixture of the sample and target binding agent and measuring a signal from the mixture, where the target binding agent is capable of assuming a first position where the target binding agent is not bound to the target polynucleotide and a second position where the target binding agent is bound to the target polynucleotide, and the intensity of the signal depends on the proportion of target binding agent in the first and second positions. The method is suitable for detecting a PCR product using a homogeneous detection method.

Method for detection of presence of target polynucleotide in samples

A reagent container having an inner surface upon which at least two reagents are dried, with the first reagent dried on a first area separate from a second area where the second reagent is dried. The first and second reagents are a nucleic acid polymerase and its substrate. A method is disclosed which includes dispensing at least the first and second reagents onto separate areas of the inner surface of the reagent container, and removing excess water from the reagents. The reagent container can be used in a polymerase chain reaction (PCR) assay, an assay that utilizes a reverse transcriptase, a reverse transcriptase polymerase chain reaction, an immuno-PCR assay, a nucleic acid sequence based assay, a proximity ligation assay, a ligase chain reaction assay, a rolling circle amplification assay, and a strand displacement amplification assay.

Method for stabilizing assay reagents, reagent container with stabilized assay reagents and use thereof

A method for rapid thermal control of a reaction volume from a preceding temperature to a target temperature includes first bringing at least the reaction vessel's second wall, which has high thermal conductivity, into direct contact with a first thermal block at a temperature higher than the target temperature if the target temperature is higher than the preceding temperature, or at a temperature lower than the target temperature if the target temperature is lower than the preceding temperature, until the reaction volume temperature is at least close to the target temperature; and then bringing the second wall into direct contact with a second thermal block at the target temperature. Also disclosed is a system () for detecting and/or quantitating a biological and/or chemical analyte in a sample and a software product for the system.

Temperature control of reaction vessel, system with reaction vessel, software product for system and use of system

The present invention relates to a homogenous method for detecting at least one target polynucleotide in a sample comprising the steps of

Homogenous method for the detection of a polynucleotide, with e.g. cleavable lanthanide chelate label

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Parainen, Finland

Jussi Nurmi