Bodenseewerk Perkin-Elmer Gmbh

Jurgen Wulf

Werner Lahmann

The present invention relates to a device for the detection of a fluorescent dye in a sample, comprising a radiation source with the aid of which light for exciting the fluorescent dye to be detected can be transmitted into the sample applied to a carrier, and a detecting device for detecting fluorescent light which has been emitted by the fluorescent dye to be detected. The present invention is characterized by a hollow space having an internal high-reflectance surface, a first aperture directed towards the sample, and a second aperture located opposite the detecting device.

Device for the detection of a fluorescent dye

The present invention relates to a device for the detection of a fluorescent dye in samples, comprising a device for exciting the fluorescent dye to be detected, said device comprising a surface provided for the application of samples and an excitation light coupling device by means of which excitation light from a predefined direction of impingement can be coupled into the device in such a way that, by means of an evanescent field of the coupled light, the fluorescent dye to be detected can be excited in the samples applied, and further comprising a means for the detection of the fluorescent light emitted by the fluorescent dye to be detected. The present invention is characterized in that the device for exciting the fluorescent dye to be detected is provided in such a way that the surface to which the samples are to be applied is movable relative to the direction of impingement so as to excite the fluorescent dye to be detected in different subsections of said surface.

The present invention relates to a light scanning device for exciting and detecting secondary light, especially fluorescent light, on a sample, comprising a light-emitting device for emitting excitation light having a wavelength which is suitable for exciting secondary light on or in the sample, a scanning unit for scanning at least one subarea of the sample with said excitation light, and a detection unit for the secondary light emitted in response to excitation of the sample, said detection unit comprising a detection optics and a detector device. In the case of conventional scanning devices, the spatial resolution on the sample is determined by the scanning element alone. If spot detectors without spatial resolution are used, the detector must be read out and re-initialized after the illumination of each scanning spot on the sample; this results in a waiting time before the scanning beam can be moved to the next scanning spot and, consequently, in a reduction of the read-out velocity. For avoiding this drawback and for increasing the read-out velocity as well as for improving the spatial resolution on the sample, the device according to the present invention makes use of a detector device comprising a large number of detection elements arranged in an array with predetermined position coordinates, said detection elements being arranged in an imaging plane of the detection optics and converting light detected in spatially resolved manner into electric signals.

Light-scanning device

The present invention refers to a dual-beam polychromator comprising a reflecting dispersion grating, a first entrance slit for a first ray bundle, a second entrance slit for a second ray bundle and first and second detector devices respectively used for simultaneously detecting the spectra of the first and second ray bundles diffracted at the dispersion grating. Dual-beam polychromators which are known according to the prior art and which have their respective entrance slits arranged directly adjacent the detector devices of the other channel cause substantial structural difficulties with regard to the mechanical provision of the respective components. In accordance with the present invention, this problem is solved by a dual-beam polychromator of the above-mentioned type, which is characterized by the features that a concave mirror means provided with an opening is located opposite the dispersion grating, said concave mirror means being adapted to be used for focussing the light, which has been diffracted at the dispersion grating, onto said first and second detector devices, and that each of the ray bundles coming from the first and second entrance slits are adapted to be directed through said opening onto the grating.

Dual-beam polychromator

A double monochromator comprises an inlet slit 20, which is imaged in the plane of an intermediate slit 34 through a first grating monochromator 36. The intermediate slit 34 is imaged in the plane of the outlet slit 34 through a second grating monochromator 58 identical with the first grating monochromator 36. Deflecting mirrors 64 and 66 can be moved into the path of rays to enable such a double monochromator to be optionally used as a single monochromator with increased light flux. An additional slit is arranged between the deflecting mirrors 64 and 66. The arrangement is such so that essentially identical beam geometries result at the outlet in both of the operation modes.

Double monochromator

A photomultiplier (10) with an elongated rectangular, cathode (20) tilted with respect to the inlet opening (14) is alternately illuminated by a measuring light beam (36) and a reference light beam (40). The measuring light beam (36) and the reference light beam (40) extend in a plane parallel to the longitudinal sides of the cathode (20) and impinge upon the cothode (20), such that they illuminate the same areas of the cathode (20).

Illuminating arrangement for illuminating a photomultiplier in a

In an atomic absorption spectrophotometer for multielement analysis, the light from a plurality of light sources each of which emits the resonance lines of one associated looked-for element is superimposed into a single light beam by means of interconnected light guides. The light is directed onto a polychromator 64 via an optical coupler.

Atomic absorption spectrophotometer for simultaneous multi-element

In a double-beam photometer comprising a light source providing a light beam, detector means, a sample area, optical means for guiding the light beam as a measuring light beam through the sample area onto the detector means, means for guiding the same light beam as a reference light beam onto the detector means while avoiding the sample area, and chopper means disposed in a splitting location for splitting the light beam into the measuring light beam, which are combined into one path of rays in a recombination location, after the measuring light beam has passed through the sample area, with the splitting location and the recombination location being spatially close to each other. The chopper means has a single chopper which simultaneously effects the splitting and the combination of the measuring and reference light beams.

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Uberlingen, Germany

Jurgen Wulf