Roche Diagnostics Operations, Inc.

Other

Hitachi-high-technologies Corporation

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Jürgen Spinke

Carina Horn

Joachim Hoenes

Dieter Heindl

Hans-Peter Josel

Taku Sakazume

Rupert Herrmann

Christoph Boehm

Manfred Augstein

Sascha Lutz

Norbert Oranth

Peter Koltay

Nadine Losleben

Timo Klein

Stefan Borja Bammesberger

Jürgen Schäffler

James Kenneth Tusa

Peter Kupser

Marcel Thiele

Laurent Tanguy

Valerie Desprez

Sabrina Kartmann

Klaus Mutschler

Andreas Nufer

Kyoko Imai

Thorsten BRÜCKNER

Christoph BÖHM

A method for the quantitative determination of an analyte in a sample is provided comprising: (a) providing an analyte-specific substance which is able to undergo a reaction which generates a detectable signal when it is contacted with an analyte; (b) providing at least two calibration graphs which have been generated by reacting in each case the same analyte-specific substance with different amounts of in each case the same test analyte for in each case a predetermined reaction time; (c) contacting the analyte-specific substance with a sample which contains the analyte to be detected; (d) measuring the signal at a first predetermined reaction time for which a first calibration graph according to (b) is provided; (c) checking whether the signal measured according to (d) enables a quantitative determination of the analyte with a desired accuracy; (f) (i) quantitatively determining the analyte on the basis of the signal measured according to (d) if the desired accuracy is reached, or (ii) measuring the signal at a second predetermined reaction time for which a second calibration graph according to (b) is provided; (g) checking whether the signal measured according to (f(ii)) enables a quantitative determination of the analyte with a desired accuracy; and (h) (i) quantitatively determining the analyte on the basis of the signal measured according to f(ii) if the desired accuracy is reached, or (ii) continuing the determination at at least one further predetermined reaction time (corresponding to (f)(ii), (g), (h)(i)).

Measuring range extension of chromatographic rapid tests

A reagent for detecting an analyte by a redox reaction and a fluorimetric determination is disclosed. The reagent comprises a compound of formula (I): wherein Rand Rare each independently selected from R, (CHCHO)R, COR, COOR, and OCOR; each Ris independently selected from NO, CN, R, OR, OCOR, COOR, SR, and halogen; R is H or C-Calkyl, where alkyl is optionally substituted with one or more functional group independently selected from the group consisting of halogen, OR, SR, NR, COOR, CONR, SOR and salts thereof, and PO(OR)and salts thereof; m is an integer from 1 to 20; and n is 1, 2, or 3. The reagent may further comprise an enzyme or a coenzyme for reducing or oxidizing an analyte.

[object Object]

The invention concerns methods and reagent kits for the fluorimetric determination of analytes.

Fluorimetric determination of analytes by amine-N-oxides as redox indicators

The present invention concerns a polymer layer for a sensor, wherein the polymer layer has nanoparticles embedded therein which impart to the polymer layer recognizing properties as well as transducer properties, a sensor comprising such layer and the use of the sensor for detecting and/or quantifying a target analyte.

Nanoparticles for optical sensors

A method and reagent for detecting an analyte by a redox reaction and a fluorimetric determination, is disclosed. The method comprises contacting a sample containing the analyte with a detection reagent which contains a compound of the general formula Q-F as a fluorimetric redox indicator, wherein Q is a quencher group and F is a fluorophore group.

Fluorimetric determination of analytes by an intramolecular quencher-fluorophore conjugate

Growing community of inventors

Lorsch, Germany

Jürgen Spinke