Hoechst Aktiengesellschaft

Jurgen Ludwig

Rainer Obermeier

Walter Sabel

Martin Gerl

Volker Teetz

Max Hropot

Dominique Tripier

Gerhard Seipke

Ingeborg Salomon

A process for isolating insulin which entails performing high-pressure liquid chromatography with pressure-stable acidic cation exchange material under a pressure of from 1.1 MPa to 40 MPa.

Process for isolating insulin by high-pressure liquid chromatography

Hirudin derivatives can be prepared by the selective enzymic replacement of a part of the molecule with an amino derivative. The hirudin derivatives are suitable for preparing pharmaceuticals having an anticoagulatory effect.

Hirudin derivatives and a process for their preparation

A process for obtaining insulin having correctly linked cystine bridges from a corresponding proinsulin amino acid chain in the presence of mercaptan, chaotropic auxiliaries and at a pH of 10 to 11 is described, as well as the direct conversion of the proinsulin to insulin with the aid of enzymes and direct isolation of the insulin from the reaction mixture with the aid of hydrophobic adsorber resins.

Process for obtaining insulin having correctly linked cystine bridges

A process is described for obtaining proinsulin possessing correctly linked cystine bridges from a corresponding proinsulin amino acid chain in the presence of mercaptan and chaotropic auxiliary agents and at a pH of 10 to 11, as well as its direct isolation from the reaction mixture using hydrophobic adsorber resins.

Process for obtaining proinsulin possessing correctly linked cystine

The invention relates to a process for the isolation and purification of .alpha.-interferons generated by plasmids from bacterial cultures which have been modified by genetic engineering, and of .alpha.-interferons from culture supernatants of induced mammalian cells, which comprises partition of appropriate crude substances containing interferon between an aqueous urea solution, which contains a surfactant, and a mixture of n-butanol and glacial acetic acid which contains water, and isolation of the purified .alpha.-interferons from the upper phase.

Process for the isolation and purification of alpha-interferons

A method for separating insulin or certain insulin derivatives from mixtures containing the same by chromatography of the mixture on a column filled with silica gel having a particle size between 40 and 500 microns with an eluant mixture comprising chloroform, methanol, water and triethylamine.

Process for the separation of mixtures of insulin, insulin derivatives

What is disclosed is a method for making human insulin or modified human insulin from pig insulin or modified pig insulin by reacting the pig insulin starting material at a pH below its isoelectric point with an excess of a threonine ester in the presence of trypsin or a trypsin-like enzyme.

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Brachttal, Germany

Jurgen Ludwig