Miles Inc.

Bayer Aktiengesellschaft

Jurgen Kocher

Klaus Wehling

Gunther Beck

Nan-Horng Lin

Robert P Hatch

Meinhard Rolf

Wolfgang Springer

Eberhard Kuckert

Scott Ruetten

The invention relates to the preparation, as substituents, of nucleotides possessing a fluorescent coumarin residue, the enzymic incorporation of these nucleotides into nucleic acids and the detection of nucleic acids of defined sequence by hybridization with a complementary, coumarin-labelled nucleic acid.

Sulphocoumarin-containing nucleotides and their use in processes for

2-Thiazolyl tetrazolium salt compounds characterized by a reflectance spectrum exhibiting an extended plateau above about 600-650 nm. Such compounds are useful as chromogenic indicators for reducing substances such as NADH. The reflectance plateau confers improved accuracy to analytical assays, particularly for the determination of analytes of medical diagnostic significance, in which a colorimetric response on a reagent carrier matrix is measured by reflectance.

Substituted 2-thiazolyl tetrazolium salt indicators

Phenyl substituted 2-thiazolyl tetrazolium salt compounds characterized by a reflectance spectrum exhibiting an extended plateau above about 600-650 nm. Such compounds are useful as chromogenic indicators for reducing substances such as NADH. The reflectance plateau confers improved accuracy to analytical assays, particularly for the determination of analytes of medical diagnostic significance, in which a colorimetric response on a reagent carrier matrix is measured by reflectance.

Phenyl substituted 2-thiazolyl tetrazolium salt indicators

Naphthotriazolium salts which are chromogenic indicators for reducing substrate, such as NADH or NADPH.

Naphthotriazolium salts

General classes of 2-thiazolyl tetrazolium salt compounds have been found to be characterized by a reflectance spectrum exhibiting an extended plateau above about 600-650 nm. Such compounds are useful as chromogenic indicators for reducing substances such as NADH. The reflectance plateau confers improved accuracy to analytical assays, particularly for the determination of analytes of medical diagnostic significance, in which a colorimetric response on a reagent carrier matrix is measured by reflectance.

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Cologne, Germany

Jurgen Kocher