Biotest Ag

Biotest-Serum-Institut Gmbh

Jurgen Horn

Hans H Sonneborn

Hans D Hempel

Wilfried Rothermel

Ullrich Muller

Heike Nebel-Schickel

Michael Becker

The present invention relates to a method for improving the growth and detection of bacteria, yeasts, fungi or cocci, by adding sterile-filtered yeast extract and/or p-iodonitrotetrazolium violet to the culture medium.

Change in system

A method of visually demonstrating the growth of such microorganisms as fungi, yeasts, and bacteria, especially mycobacteria, identifying them, and testing them for sensitivity to antibiotics by the color change of a specific redox indicator system. A mixture of the redox indicators Methylene Blue and resazurin is added to the culture medium. Iron(III) salts mixed with K.sub.3 Fe(CN).sub.6, Iron(II) salts mixed with K.sub.4 Fe(CN).sub.6, or sodium tungstate (Na.sub.2 WO.sub.4) is added to the culture medium.

Method of visually demonstrating the presence of microorganisms,

The invention concerns a method of detecting proteins, especially HIV and CMV antibodies in body fluids using solid-phase immune reaction with a buffer containing an animal serum and/or a non-ionic surfactant in a phosphate or carbonate buffer together with further additives selected from inorganic salts, water-soluble polymers, organic compounds, and propionic acid and their derivatives and mixtures. The buffer makes it possible to reliably eliminate false positive results.

Method of detecting proteins in body fluids and means of carrying out

A blood-culture flask with an integrated subculture. It comprises a flask proper that can be filled with a liquid nutrient solution and has a neck with an outside thread, a support that can be coated with a solid culture medium, a resilient stopper with an edge that projects at one end, and a screw cap. The screw cap consists of a bottom that is open above and below and has an inside thread and of a top that is open below. The inside thread is dimensioned so that it can be screwed down over the outside thread on the neck of the flask so that the upper edge of the thread is below the upper edge of the neck when screwed on. The resilient stopper is positioned inside the bottom of the screw cap so that the bottom surface of its resilient projecting edge comes to rest against the offset above the thread when not screwed on and its lower section is dimensioned so that it can be introduced tight into the neck of the flask. The support has two surface that can be coated with a solid culture medium on opposite sides, is fastened to the bottom of the resilient stopper, and extends in use only over part of the height of the flask, and the top of the screw cap is designed so that in assembled state it forces the resilient projecting edge of the stopper tightly against the upper edge of the neck of the flask.

Growing community of inventors

Egelsbach, Germany

Jurgen Horn