Medeva Pharma Limited

Juraj Ivanyi

Pavel Novotny

Juan Antonio Crespo

Juan Antonio Montaraz Crespo

Pavel Novotny, Deceased

By Jaroslava Novotny, Administrator

Jaroslava Novotny

The present invention provides novel antigenic preparations comprising proteinaceous material associated with adenylate cyclase activity in cultures of,, the said preparations being useful as components of acellular whooping cough vaccines. The invention further provides methods for the isolation of such antigenic preparations.

Antigenic preparations and isolation of such preparations

A method of deriving an antigen from Bordetella pertussis, wherein the antigen is characterized by the following features: a relative molecular weight of about 67,000 to 73,000 as determined by 12%(w/w) polyacrylamide gel electrophoresis and a proline:glutamic acid ratio of about 1:1 as determined by amino acid analysis. The method comprises: (a) culturing Bordetella pertussiscells; (b) treating the culture of (a) to obtain an outer membrane fraction; and (c) isolating the antigen from the outer membrane fraction of (b).

Antigenic preparations and the isolation of such preparations

The invention provides a vaccine containing a proteinaceous material derived from the outer membrane of Bordetella pertussis, wherein the proteinaceous material is characterized by a relative molecular weight of about 67,000 to 73,000, preferably 69,000, as determined by 12% (w/w) polyacrylamide gel electrophoresis and a proline:glutamic acid ratio of about 1:1 as determined by amino acid analysis, in a pharmaceutically acceptable carrier or adjuvant. The invention also provides a method of inducing an immune response in an individual involving administering the vaccine of the invention to an individual.

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Juraj Ivanyi