Kaneka Corporation

Kanegafuchi Kagaku Kogyo Kabushiki Kaisha

Kanegafuchi Chemical Industry Co., Ltd.

Junzo Hasegawa

Yoshihiko Yasohara

Noriyuki Kizaki

Masahiro Ogura

Shigeru Kawano

Yasuhiro Ikenaka

Kenji Miyamoto

Kiyoshi Watanabe

Yukio Yamada

Akira Iwasaki

Hajime Kawaharada

Satomi Takahashi

Shigeki Hamaguchi

Ikuo Sawa

Sakayu Shimizu

Kazuyoshi Yajima

Noboru Ueyama

Natsuki Mori

Masaru Wada

Michihiko Kataoka

Hideyuki Matsuda

Makoto Kawamukai

Shunichi Maemoto

Hidetoshi Kutsuki

Kazuhiko Yamamoto

Keiko Kita

Hiroshi Kawabata

Hamao Umezawa

Kenji Inoue

Masakatsu Shibasaki

Keiji Matsumoto

Takehisa Ohashi

Masaru Mitsuda

Nobuo Nagashima

Tadayoshi Shiraishi

Masaji Ohno

Masahiko Yamada

Hideyuki Takahashi

Shigeo Hayashi

Masami Shimazaki

Miho Horikawa

Kenichi Nishi

Yuko Konishi

Shunich Maemoto

Yoshiko Tari

Masura Mitsuda

Hiroshi Kanema

Hiroshi Yamamura

The present invention relates to a method of producing an optically active pyridineethanol derivative. More particularly, it relates to a method of producing an optically active polycyclic pyridineethanol derivative by causing an enzyme or enzyme source to act on polycyclic acetylpyridine derivatives. The present invention also relates to a novel enzyme which can be used in the production method mentioned above, a DNA coding for said enzyme, a recombinant vector having said DNA, and a transformant having said recombinant vector. The invention further relates to a method of producing an optically active polycyclic pyridineethanol derivative by causing the above novel enzyme or the above transformant to act on optically inactive polycyclic pyridineethanol derivatives.

Enzyme for producing optically active pyridineethanol derivatives

To provide a method for preparing optically active compounds, which mainly contain (R)-amino compounds, by microbial enzymes efficiently and inexpensively; a polypeptide having stereoselective transaminase activity which can be suitably used for the above preparation method; and a DNA encoding the polypeptide. A method for preparing an optically active amino compound, characterized in stereoselectively transaminating by acting a transaminase on an amino group acceptor, a ketone compound in the presence of an amino group donor, a primary amine; a DNA comprising a nucleotide sequence encoding a polypeptide having stereoselective transaminase activity; and a polypeptide having stereoselective transaminase activity obtainable from a culture of a microorganism belonging to the genus Arthrobacter.

Producing optically active amino compounds

The present invention provides a novel polypeptide producing (S)-N-benzyl-3-pyrrolidinol, a DNA coding for it and a method of using them. A polypeptide having the following physicochemical properties (1) to (5): Further, a polypeptide having the amino acid sequence shown under SEQ ID NO:1 in the sequence listing; or a polypeptide having an amino acid sequence obtainable from the amino acid sequence shown under SEQ ID NO:1 in the sequence listing by substitution, insertion, deletion and/or addition of one or more amino acids and having enzyme activity in asymmetrically reducing N-benzyl-3-pyrrolidinone to produce (S)-N-benzyl-3-pyrrolidinol.

Carbonyl reductase, gene thereof and method of using the same

Process for producing coenzyme Q10

A novel polypeptide for producing tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate, a gene encoding the polypeptide, and a method of using the polypeptide, are provided. The polypeptide has an enzyme activity to asymmetrically reduce tert-butyl (S)-6-chloro-5-hydroxy-3-oxohexanoate to tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate. The polypeptide comprises the amino acid sequence represented by SEQ ID NO. 1 in the Sequence Listing, or the amino acid sequence having at least one amino acid substitution, insertion, deletion, or addition thereof.

A gene of decaprenyl diphosphate synthase, which is the key gene participating in the biosynthesis of coenzyme Q was isolated from a bacterium belonging to the family Rhizobiaceae. By transferring this gene into a microorganism such as and expressing therein, coenzyme Q can be effectively produced.

An enzyme having carbonyl reduction activity of reducing a carbonyl compound asymmetrically to produce an optically active alcohol, a DNA coding the enzyme, a plasmid having the DNA, a transformant which is a cell transformed with the plasmid, and a production method of an optically active alcohol using the enzyme and/or the transformed cell are provided.

Carbonyl reductase enzyme and methods for its use

Novel and useful optical active 2-aralkyl-3-sulfonyloxy-1-propanol and 2-aralkyl-3-sulfonyloxypropionic acid are provided by using an optical active 2-aralkyl-3-acyloxy-1-propanol as a starting material. Furthermore, an optical active 2-aralkyl-3-thiopropionic acid, which is an important intermediate of enkephalinase inhibitor, is provided. According to the present invention, industrially useful optical active sulfonic acid ester derivatives can be provided.

Sulfonic acid ester derivatives, method for production thereof and use thereof

Relating to an enzyme capable of efficiently converting a ketone compound to an optically active amino compound by transamination, and a process for preparing an optically active amino compound using the enzyme. An (S)-&agr;-phenethylamine:pyruvate transaminase, which acts on (S)-&agr;-phenethylamine and a ketone compound, thereby catalyzing transamination for forming acetophenone and an amino compound corresponding to the ketone compound; a process for preparing an optically active amino compound using the transaminase; and a method for culturing a microorganism for producing the above transaminase, comprising adding to a medium one or more compounds selected from the group consisting of propylamine, 1-butylamine, 2-butylamine, 2-pentylamine, isopropylamine and isobutylamine as an inducer for the enzyme when a microorganism for producing (S)-&agr;-phenethylamine:pyruvate transaminase is cultured.

(S)-&agr;-phenethylamine: pyruvate transaminase

The method for preparing an optically active (R)-amino compound characterized by the method comprising stereoselectively carrying out amino group transfer by action of an (R)-form-specific transaminase in the co-presence of a ketone compound (amino acceptor), and an amino compound (amino donor) of a racemic form or an (R)-form, to give an optically active (R)-amino compound. According to the present invention, it is made possible to easily prepare at a high yield the optically active (R)-amino compounds and the like having an aryl group and the like at their 1-position, which have been conventionally difficult to prepare.

(R)-transaminase from arthrobacter

Process for producing optically active amino compounds

Gene encoding carbonyl reductase, and methods for its use

Process for the preparation of optically active N-benzyl-3-pyrrolidinol

Sulfonic ester derivatives, process for preparing the same, and use

A novel glycerol derivative, a process for preparing the same, and a process for preparing a triazole derivative. An optically active 2-arylglycerol derivative which is novel and useful as a synthetic intermediate of medicament is provided, and furthermore, (R)-2-(2,4-difluorophenyl)-3-(1H-1,2,4-triazole-1-yl)-propane-1,2-diol, which is useful as an antifungal agent, is prepared.

Diacyloxy propanol derivatives

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Akashi, Japan

Junzo Hasegawa