Korea Advanced Institute of Science and Technology

Junyoung Seo

Young-Gyu Yoon

Hyunwoo Kim

Jae-Byum Chang

JaeByum Chang

Yeonbo Sim

Hoyeon Nam

Jee Won Kim

Junmo Cho

Jae-Byum Jang

Seoungbin Bae

The present disclosure relates to a method and device for unmixing fluorescent signals based on a joint histogram. The method and device may be configured to obtain a plurality of images of a plurality of fluorescent materials marked in different biomolecules and to unmix the signals of the fluorescent materials based on at least one joint histogram index defined between the images of each of pairs each including two or more of the obtained images. According to the present disclosure, the joint histogram index may include at least one of Kullback-Leibler divergence, cross-entropy, or Rand-index.

Joint histogram based fluorescent signal unmixing method and device

As an aspect of the present disclosure, a method of processing an image may be proposed. The method is a method of processing an image, which is performed by an electronic device including one or more processors and one or more memories in which instructions to be executed by the one or more processors are stored, and may include obtaining a first mixed image of a sample including a first biomolecule labeled with a first fluorescent material and a second biomolecule that has not been labeled, obtaining a second mixed image of the sample including the first biomolecule labeled with the first fluorescent material and the second biomolecule labeled with a second fluorescent material, and generating an unmixed image of the second biomolecule based on the first mixed image and the second mixed image.

Apparatus and method for processing image

Methods and apparatuses for multicolor unmixing may involve iterative mutual information minimization in a simultaneous channel update structure. A plurality of images may be obtained with respect to a plurality of fluorophores labeled on different biomolecules, respectively. Images of the fluorophores may be unmixed from the obtained images, respectively, while simultaneously minimizing mutual information shared between the obtained images based on all permutation combinations of the obtained images. Signals having three or more fluorophores whose emission spectra are overlapped in one spectral range can be more accurately unmixed.

Method and apparatus for multicolor unmixing through iterative mutual-information minimization in simultaneous channel update structure

The present disclosure provides a method and apparatus for biomolecular multiplexed imaging through the iterative unmixing of fluorophores. According to the first embodiments of the present disclosure, although signals of two fluorophores are detected in the first fluorescent detection spectral range, the signals of the two images obtained from two detection spectral ranges can be unmixed through the iterative minimization of mutual information. Furthermore, the present disclosure provides a multi-color unmixing method and apparatus through the iterative minimization of mutual information. In the second embodiments of the present disclosure, a plurality of images of a plurality of fluorophores marking different biomolecules, respectively, for example, N fluorophores are obtained. Images, each containing the signals of single fluorophore can be obtained from the obtained images while minimizing the mutual information shared by images of each of pairs each consisting of two of the obtained images.

Method and apparatus for multiplexed imaging of biomolecule through iterative unmixing of fluorophore signals

Multiplexed fluorescent imaging which is essential for finding out how various biomolecules are spatially distributed in cells or tissues is disclosed. The present disclosure may obtain 10 or more different biomolecule images with one labeling and imaging by newly designing selection of fluorophores, detection spectral ranges, and signal unmixing algorithm. The present disclosure is a blind unmixing technology for unmixing an image without an emission spectrum of fluorophore, and in this technology, 4 pairs of fluorophores are used, and each pair consists of two fluorophores in which emission spectra are overlapped. Each pair of fluorophores is strongly excited by only one excitation laser. Two images with different detection spectral ranges are obtained for each pair, and two images are unmixed via mutual information minimization without fluorophore emission spectrum information. Two images also may be unmixed via Gram-Schmidt orthogonalization and fluorescence measurement based unmixing. This signal unmixing is repeated for each pair of fluorophores. Furthermore, a total of 10 or more fluorophores may be simultaneously used by adding two large stoke's shift fluorophores emitting light in wavelength ranges that does not overlap with the emission spectra of the above 8 fluorophores.

Growing community of inventors

Daejeon, South Korea

Junyoung Seo