Wisconsin Alumni Research Foundation

Cellular Dynamics International, Inc.

Fujifilm Cellular Dynamics, Inc.

Junying Yu

James A Thomson

Maksym A Vodyanyk

Fongching Kevin Chau

Jinlan Jiang

Yong Jiang

Igor I Slukvin

Deepika Rajesh

Sarah Burton

Lisa Harms

Ying Zhang

Jeffrey Sasaki

Sarah A Burton

The present invention relates to methods for reprogramming a somatic cell to pluripotency by administering into the somatic cell at least one or a plurality of potency-determining factors. The invention also relates to pluripotent cell populations obtained using a reprogramming method.

Reprogramming somatic cells into pluripotent cells using a vector encoding Oct4 and Sox2

The present disclosure generally regards methods and compositions for providing multi-lineage hematopoietic precursor cells from pluripotent stem cells (PSCs). The PSCs comprise an expression construct encoding an ETS/ERG gene, GATA2 and HOXA9. Also provided are methods for providing hematopoietic stem cells capable of long-term engraftment in mammals, such as humans. Further provided are therapeutic compositions including the provided hematopoietic stem cells and precursors of hematopoietic cells, and methods of using such for the treatment of subjects.

Multi-lineage hematopoietic precursor cell production by genetic programming

Somatic cell reprogramming

Provided herein are methods for the generation of functional keratinocyte stem cells that are differentiated directly from human ESCs/iPSCs in a chemically defined serum-free cell culture system, as well as cells derived therefrom and methods of use thereof. Also provided are methods for culturing primary keratinocytes.

Generation of keratinocytes from pluripotent stem cells and maintenance of keratinocyte cultures

The invention generally regards methods for providing hematopoietic cells and precursors of hematopoietic cells from a variety of cell sources, such as pluripotent stem cells or somatic cells. Also provided are therapeutic compositions including the provided hematopoietic cells and precursors of hematopoietic cells, and methods of using such for the treatment of subjects.

Hematopoietic precursor cell production by programming

Enriched population of human pluripotent cells with Oct-4 and Sox2 integrated into their genome

The invention generally regards methods for providing endothelial cells and precursors of endothelial cells from a variety of cell sources, such as pluripotent stem cells. Also provided are therapeutic compositions including the provided endothelial cells, and methods of using them for the treatment of subjects.

Endothelial cell production by programming

Methods and composition of induction of pluripotent stem cells are disclosed. For example, in certain aspects methods for generating essentially vector-free induced pluripotent stem cells with cell signaling regulators are described. Furthermore, certain aspects of the invention provide novel compositions comprising induced pluripotent stem cells essentially free of exogenous retroviral vector elements in the presence of a medium comprising signaling inhibitors. In certain aspects, feeder-free episomal reprogramming methods may be provided.

Episomal reprogramming with chemicals

The invention generally features methods for providing hepatocytes from a variety of cell sources, particularly pluripotent stem cells, therapeutic compositions featuring such cells, and methods of using them for the treatment of subjects.

Hepatocyte production by forward programming

Reprogramming somatic cells using retroviral vectors comprising Oct-4 and Sox2 genes

Methods for reprogramming primate somatic cells to pluripotency using an episomal vector that does not encode an infectious virus are disclosed. Pluripotent cells produced in the methods are also disclosed.

OCT4 and SOX2 with SV40 T antigen produce pluripotent stem cells from primate somatic cells

Composition comprising recombinant nucleic acid encoding Sox2, Oct-4, Nanog and Lin28

Methods for obtaining multipotent mesenchymal stem cells under serum-free conditions and methods for identifying multipotent mesenchymal progenitor cells are disclosed.

Growing community of inventors

Madison, WI, United States of America

Junying Yu