Jcr Pharmaceuticals Co., Ltd.

Junya Tani

Kenichi Takahashi

Takashi Hanada

Atsushi Sugimura

Kazutoshi Mihara

Shinji Kakimoto

Miroslav Matev

Hidehito Yasukawa

Tsuyoshi Fukui

Shinji Okabe

Yukichi Hatano

Yuuka Asano

An aqueous preparation containing a protein as an active ingredient which is stable in storage in solution and makes an injection pain reduced is provided. More specifically an aqueous preparation containing a phosphate buffer at a concentration of 1 to 20 mM and a protein as an active ingredient is provided. Further more specifically provided is an aqueous preparation containing a phosphate buffer at a concentration of 1 to 20 mM, human growth hormone as an active ingredient, a poloxamer as a non-ionic surfactant; and phenol as a isotonic agent.

Protein-containing aqueous liquid formulation

Disclosed is a highly efficient method for production of highly pure mutant-type human erythropoietin. The method is for production of mutant-type human erythropoietin, in which a transformed mammalian cell is allowed to produce the mutant-type human erythropoietin, and the supernatant of the culture is subjected to hydrophobic column chromatography, multimodal anion exchange column chromatography, anion exchange column chromatography, phosphate group affinity column chromatography, and gel filtration column chromatography, in this order.

Method for production of mutant-type human erythropoietin

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Kobe, Japan

Junya Tani