Cansino Biologics Inc.

Junqiang Li

Tao Zhu

Zhongqi Shao

Xuefeng Yu

Mingming Yang

Jin Ma

Lei Duan

Helen Huihua Mao

Rong Jiang

Weiwei Chen

Danqing Miao

Haiyan Cui

Chunlin Xin

Huihua Mao

Provided are a cell strain HEK293.CS for reducing the production of a replication competent adenovirus, and a construction method and the use thereof. HEK293.CS is a safe adenovirus-producing cell line constructed by knocking out a gene fragment homologous to the Ad5 adenovirus E1 gene in HEK293 and providing a template plasmid to replace said gene fragment with a non-homologous sequence that stabilizes the expression of the E1 gene. Compared with the unmodified HEK293 cell strain, HEK293.CS shows no decrease in growth ability and virus production ability, but does not produce a detectable RCA. HEK293.CS can be used for the mass culture of a recombinant human type 5 adenovirus, and reducing the probability of RCA production in the manufacture process of drugs such as vaccines and antibodies.

Cell strain for reducing production of replication competent adenovirus, and construction method and use thereof

Provided are a fusion protein and a construction method thereof. The fusion method consists of: aprotein D and a Hin47 (Htra) protein. The fusion protein can serve as a protein vehicle for apolysaccharide-protein conjugate vaccine, thereby increasing immunogenicity of a polysaccharide antigen.

fusion protein and construction method and use thereof

A method for enhancing immunogenicity of an epitope peptide of an HPV antigen, the method including: assembling a gene of an HPV antigen into a gene of HBc, exogenously expressing a resulting assembled gene to acquire a fusion protein, allowing the fusion protein to automatically assemble to form a virus-like particle including the HPV antigen on a surface of the virus-like particle, to obtain HBc-HPV virus-like particle. A virus-like particle capable of expressing the antigen peptide of HPV acquired by the method. The virus-like particle includes a HBc-L2 fusion protein. A genome for encoding the HBc-L2 fusion protein is represented by SEQ ID NO. 3. A method for preparing an HPV vaccine includes using the virus-like particle.

Method for enhancing immunogenicity of epitope peptide of HPV antigen, virus-like particle, and method for preparing HPV vaccine

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Tianjin, China

Junqiang Li