Takara Shuzo Co., Inc.

Takara Bio Inc.

Junko Yamamoto

Hiroyuki Mukai

Kiyozo Asada

Kazutoh Takesako

Katsushige Ikai

Teruya Nakamura

Kazuo Shimanaka

Hideyo Yamaguchi

Fumiyo Haruna

Katsuhisa Uchida

Ikunoshin Kato

Takashi Uemori

Kazue Miyake

Fumitsugu Hino

Keiko Kubo

Hiroaki Sagawa

Eiji Kobayashi

Tatsuji Enoki

Jun Tomono

Yoshimi Sato

Michio Hagiya

Osamu Takeda

Toshiharu Ohba

Tooru Suzuki

Mariko Moriyama

Haruhisa Sawaragi

Tomoo Inden

The present invention pertains to a method for modifying a nucleic acid contained in a sample comprising a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, comprising the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.

Method for modifying nucleic acids

The present invention pertains to: a method for modifying a nucleic acid contained in a sample, the method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, the method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.

An object of the present invention is to provide a simple and useful method for producing an RNA-containing probe for detecting a target nucleotide, a simple and useful method, device, and system for processing nucleotide sequence information, and a simple and useful method for detecting a target nucleotide. The present invention provides a method for processing nucleotide sequence information, the method comprising the step of generating partial nucleotide sequences which has 7 to 14 nucleotides and a Tm value of 25 to 40&#xb0; C. and in which a target nucleotide or a nucleotide adjacent to the target nucleotide is located at a position between 3 and 5 nucleotides from the 3' or 5&#x2032; end. The method according to the present invention is useful for simply and efficiently producing an RNA-containing probe for detecting a target nucleic acid, without the basis of researchers' experiences or guess, and are extremely useful not only in the field of genetic engineering, but also in the field of medical research.

Method for producing RNA-containing probe for detecting a target nucleotide

A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.

Method for amplifying nucleic acid sequence

A method for synthesizing cDNA characterized by performing a reverse transcription reaction in the presence of an enzyme having a reverse transcriptional activity and another enzyme different from the former one which as a 3'-5&#x2032; exonuclease activity.

Method for synthesizing cDNA

A method for synthesizing cDNA characterized by performing a reverse transcription reaction in the presence of an enzyme having a reverse transcriptional activity and another enzyme different from the former one which as a 3&prime;-5&prime; exonuclease activity.

An antibiotic R106 represented by the general formula I): ##STR1## wherein: R is methyl or ethyl;

Aureobasidium pullulans which produces antibiotic R106

There are provided R106 compounds represented by the following general formula (I): ##STR1## wherein: A.sub.1 is Phe, o-FPhe, m-FPhe, or Tyr;

R106 compounds

An antibiotic R106 represented by the general formula (I): ##STR1## wherein: R is methyl or ethyl;

Process for the production of antibiotic R106 by a strain of

Novel antibiotics R106

Growing community of inventors

Moriyama, Japan

Junko Yamamoto