Panasonic Corporation

Panasonic Intellectual Property Management Co., Ltd.

Junko Wakai

Akihito Kamei

Jin Muraoka

Using a biopanning method, a heat-stable antibody-displayed phage is acquired. More particularly, first, an antibody-displayed phage library aqueous solution containing plural types of antibody-displayed phages is supplied to a support comprising a polypeptide on the surface thereof, so as to bind the plural types of the antibody-displayed phages to the polypeptide specifically. Next, the support is heated to the temperature of not less than 37 degrees Celsius and not more than 70 degrees Celsius, so as to release a portion of the antibody-displayed phages from the support and so as to leave the other antibody-displayed phages on the support selectively. Finally, the other antibody-displayed phages which has been left on the support selectively in the previous step is collected to obtain the heat-stable antibody-displayed phage.

Method for acquiring a heat-stable antibody-displayed phage

Provided is an immunoassay method that can reduce the effort of establishing an immunoassay system due to not needing two or more antibodies, and that is applicable to not only high molecular weight antigens but also to low molecular weight antigens such as hapten. The method is for releasing, from a surface of a base plate, immunoglobulin G antibody bound to the surface via protein A. The method includes the following steps (A) and (B): (A) a step of preparing the base plate having the surface to which immunoglobulin G antibody produced by a producer cell line of deposit No. FERM BP-10459 is bound via protein A; and (B) a step of providing a solution (from pH 6 to pH 8.9; preferably, from pH 7.4 to pH 8.9) containing human serum albumin onto the surface so as to release the immunoglobulin G antibody from the protein A.

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Osaka, Japan

Junko Wakai