Mitsui Chemicals, Inc.

Mitsui Toatsu Chemicals, Incorporated

Junko Tokuda

Daisuke Mochizuki

Hitoshi Takahashi

Takashi Morishige

Mitsufumi Wada

Tadashi Araki

Tadashi Suzuki

Nobuo Kato

Hideki Sawai

Takashi Mimitsuka

Yasuyoshi Sakai

Takeshi Nakamura

Toshihiro Oikawa

Kazuya Matsumoto

Hitoki Miyake

Katsuyuki Takahashi

Yosuke Higashi

Masao Shimada

Reiko Abe

Miyuki Kawashima

Shin-ichirou Tawaki

Toshihiro Tateno

Yasushi Kazuno

Keiichirou Kai

Tomoyuki Natsuji

Mitsufumi Wade

A mutant nitrile hydratase that is derived fromand has an &#x3b1; subunit and a &#x3b2; subunit, wherein a specific amino acid residue has been substituted for the amino acid residue in at least one position selected from the group consisting of the 40th and 43rd residues from the N terminal of the &#x3b1; subunit, and the 205th, 206th, and 215th residues from the N terminal of the &#x3b2; subunit.

Mutant nitrile hydratase, nucleic acid coding said mutant nitrile hydratase, expression vector and transformant including said nucleic acid, production method for said mutant nitrile hydratase, and production method for amide compound

Disclosed is anproducing 2-deoxy-scyllo-inosose (DOI), which, from a sucrose non-PTS gene group, has at least a sucrose hydrolase (CscA)-encoding gene and which is provided with a DOI production system or has an enhanced DOI production system. Thepreferably further includes a system to enhance sugar uptake capacity. There is also disclosed a method of producing DOI from a plant-derived raw material containing sucrose by using the

Bacterium producing 2-deoxy-scyllo-inosose (DOI) and method of producing 2-deoxy-scyllo-inosose (DOI) by using same

Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to produce nicotinamide adenine dinucleotide by deleting, mutating or substituting nadR gene in the microorganism or introducing a gene encoding nicotinic acid phosphoribosyltransferase.

Method for producing hydroxycarboxylic acid by enhancing synthesis of coenzyme

A nitrile hydratase variant of the present invention comprises substitution of at least one amino acid with another amino acid to improve two or more properties of nitrile hydratase by substitution of one amino acid.

Nitrile hydratase variant

Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to regenerate oxidized-type nicotinamide adenine dinucleotide by being provided with an enhanced NADH dehydrogenase function by introducing a gene encoding NADH dehydrogenase into a microorganism.

Method for producing hydroxycarboxylic acid by regenerating coenzyme

A method for producing D-lactic acid in high yield, and to provide a method for producing D-lactic acid with high selectivity, in which optical purity is high and a by-product organic acid is small. In one aspect, a microorganism, wherein activity of pyruvate formate-lyase (pfl) is inactivated or decreased, and further activity of-derived NADH-dependent D-lactate dehydrogenase (ldhA) is enhanced, is cultured to efficiently produce D-lactic acid. With regard to a method for enhancing ldhA activity, by linking, on a genome, a gene encoding ldhA with a promoter of a gene which controls expression of a protein involved in a glycolytic pathway, a nucleic acid biosynthesis pathway or an amino acid biosynthesis pathway, suitable results are obtained compared to the method for enhancing expression of the gene using an expression vector. A microorganism in which a dld gene is substantially inactivated or decreased is cultured to produce high quality D-lactic acid with reduced concentration of pyruvic acid.

Biocatalyst for production of D-lactic acid

A method for producing phytase using a Candida boidinii transformant, by which phytase is secreted to liquid medium without substantially increasing the number of the transformant cells is disclosed. In this method, cells of a Candida boidinii transformant which is transformed with a recombinant vector comprising a promoter inducible with methanol and a gene located downstream of said promoter and operably linked to said promoter, which gene encodes a polypeptide having phytase activity, are cultured in a liquid culture medium containing phosphoric acid or a phosphate compound in an amount effective of restricting proliferation of said cells of Candida boidinii transformant such that the amount of said cells of Candida boidinii transformant is not substantially increased, while adding methanol to the culture medium, so as to make said cells secrete said phytase.

Method for producing phytase

A novel phytase is disclosed. The phytase consists essentially of a single type of subunits, and can hydrolyze a phytate to myo-inositol. A gene encoding the phytase, which is originated from Schwanniomyces occidentalis is also disclosed.

Phytase

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Chiba, Japan

Junko Tokuda