Ajinomoto Co., Ltd.

Junko Shinozaki

Kenzo Yokozeki

Seiichi Sato

Koichi Ishii

Shunichi Suzuki

Naoyuki Fukuchi

Morikazu Kito

Shigeru Yamanaka

Yasuko Jojima

Yukitsugu Ono

Akiko Tanaka

Katsunori Kobayashi

Fumie Futaki

Yuko Tanita

Naoko Tsuyoshi

Ryosuke Fudo

Takayuki Kajiura

A method for conveniently detecting binding between the von Willebrand factor and glycoprotein Ib and a means to be used therein. The von Willebrand factor fixed in a reactor immobilized in a reaction vessel in the presence of bottrocetin is bound to a chimeric protein constructed by fusing the carboxyl terminal of a partial protein containing the von Willebrand factor-binding site of glycoprotein Ib with the amino terminal of the Fc region of an immunoglobulin molecule. Then the Fc region of the above immunoglobulin molecule is detected to thereby detect the binding between the von Willebrand factor and the glycoprotein Ib or inhibition of this binding.

Substance with antithrombotic activity and method for detecting glycokallidin

The present invention provides a process for producing a transglutaminase, which comprises culturing microorganisms of any of the genus Micrococcus, Clostridium, Torulopsis, Rhizopus and Monascus in a medium to produce the intended transglutaminase in the medium or in the cells of the microorganisms and then isolating the transglutaminase, and a process for producing a gelled proten with the thus-obtained transglutaminase. According to the process of the present invention, transglutaminase can be rapidly produced at a low cost.

Process for producing transglutaminase by microorganism

Growing community of inventors

Kawasaki, Japan

Junko Shinozaki