Arkray, Inc.

Ultizyme International Ltd.

I-sens, Inc.

Junko Shimazaki

Koji Sode

Kazushige Mori

Katsuhiro Kojima

Koji Katsuki

Hisashi Kaneda

Yuka Takahashi

Yosuke Murase

Masashi Tsukada

Youngjea Kang

Megumi Saeda

The purpose of the present invention is to provide a fungus-derived FADGDH that has a direct electron transfer ability. Provided is a fusion protein comprising a fungus-derived FADGDH or a mutant thereof and a cytochrome molecule connected to the N-terminus of the FADGDH or variant thereof.

Fusion protein of flavin adenine dinucleotide-glucose dehydrogenase and cytochrome molecule

A mutant cytochrome protein originated from a cytochrome protein having three heme-binding domains, which mutant cytochrome protein lacks the first heme-binding domain and the second heme-binding domain as counted from the N-terminus, is provided. The mutant cytochrome protein may lack a region(s) containing the first and second heme-binding domains.

Mutant cytochrome protein lacking certain heme domains and use thereof

A molecular recognition element comprising a target molecule-recognizing portion, and a direct electron transfer-type oxidoreductase linked to the target molecule-recognizing portion.

Direct electron transfer-type oxidoreductase-modified molecular recognition element

A biosensor includes a plurality of electrodes including a working electrode, and a detection layer containing an enzyme for exchanging electrons with the working electrode, a crosslinking agent and an electrically conductive polymer and having a contact area with the working electrode defined by a predetermined area.

Biosensor and manufacturing method of biosensor

Mutant cytochrome protein and use thereof

An enzyme electrode includes an electrode, and a detection layer which contacts the electrode and contains a crosslinking agent, an electrically conductive macromolecule and an enzyme transferring and receiving electrons to and from the electrode and does not contain an electron transfer subunit.

Enzyme electrode

The disclosure discloses an enzyme electrode comprising; an electrode comprising a current collector; a monolayer-forming molecule bound to the surface of the current collector; and a glucose dehydrogenase comprising a cytochrome C subunit bound to the monolayer-forming molecule; wherein electrons are transferred between the glucose dehydrogenase and the current collector by oxidation-reduction reaction of the glucose dehydrogenase.

Enzyme electrode and biosensor using the same

A biosensor includes a plurality of electrodes including a working electrode, and a detection layer which is immobilized on the working electrode and contains a crosslinking agent, an electrically conductive macromolecule and an enzyme transferring and receiving electrons to and from the working electrode. The working electrode is in an open system.

Growing community of inventors

Tokyo, Japan

Junko Shimazaki