Life Technologies Corporation

Applied Biosystems, LLC

Junko F Stevens

Shoulian Dong

Carole Bornarth

Michael Lau

Danny H Lee

Chunmei Liu

Kenneth J Livak

Emily S Winn-Deen

Eugene G Spier

Yu Wang

Luz Montesclaros

Jeremy Heil

Ivy McMullen

Ryan T Koehler

Cora L Woo

Francisco M De La Vega

Michael Rhodes

Leila G Smith

Laurent R Bellon

Dawn Madden

Dennis A Gilbert

Xiaoqing You

Lily Xu

Stephen A Glanowski

Julie Williams

Susan Eddins

The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

Compositions, methods and kits for real time polymerase chain reaction (PCR)

The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided.

Compositions, methods, and kits for amplifying nucleic acids

Methods and systems for ordering assays which detect SNPs or gene expression are provided. The methods use PCR and RT-PCR procedures. Collections of stock assays are assembled using pre- and post-manufacturing quality control procedures and made available to consumers via the Internet. In addition, custom assays are prepared upon order from the consumer and these assays are also prepared using pre- and post-manufacturing quality control procedures. The assays are then delivered to the consumer.

Methods for placing, accepting, and filling orders for products and services

Disclosed herein are compositions, methods and kits for analyzing three-dimensional chromatin and/or chromosome conformation. Method are also disclosed for using the methods disclosed herein for diagnosing diseases such as cancer.

Chromosome conformation analysis

The present teachings provide DNA methylation quantification methods that avoid bisulfite treatment of DNA. Methylation-specific binding proteins (MeDNA binding proteins) and non-methylation specific binding proteins (non-MeDNA binding proteins) are employed in various embodiments to modulate the accessibility of nucleic acids to primer extension reactions. After selectively removing the target nucleic acids, the extension products can be analyzed and methylation quantitated. In some embodiments, the analysis comprises real-time PCR.

DNA methylation detection methods

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Menlo Park, CA, United States of America

Junko F Stevens