Spiber Inc.

Riken Corporation

Kojima Industries Corporation

The Systems Biology Institute

Junichi Sugahara

Keisuke Morita

Keiji Numata

Kazuhide Sekiyama

Kaori Sekiyama

Mizuki Ishikawa

Yunosuke Abe

Shinya Murata

Nozomu Yachie

Ayumi Abe

Takehisa Maekawa

Hiroyuki Nakamura

Junichi Shimokata

Ryota Sato

Kazuko Otomo

Kousuke Tsuchiya

Junichi Noba

Koichi Kotaka

Takaoki Ishii

Shinji Hirai

Hongfang Chi

The present invention relates to a highly contracted artificial fibroin fiber including a modified fibroin, in which a contraction percentage defined by the following equation exceeds 7%.Contraction percentage={1−(length of contracted artificial fibroin fiber/length of artificial fibroin fiber before being brought into contact with water after spinning)}×100(%)

Highly contracted synthetic fibroin fiber, production method therefor, and method for contracting synthetic fibroin fiber

The present invention relates to a modified fibroin including a domain sequence represented by Formula 1: [(A)motif-REP]or Formula 2: [(A)motif-REP]&#x2212;(A)motif, in which the domain sequence has an amino acid sequence with a reduced content of a glutamine residue equivalent to an amino acid in which one or a plurality of glutamine residues in REP are deleted or substituted with other amino acid residues, as compared with a naturally occurring fibroin.

Modified fibroin

An encryption method includes an operation method of an encryption system and is a method of encrypting encryption target information.

Encryption method, decryption method, encryption system and decryption system

Provided are a composite molding composition such as a composite film and a composite fiber that have properties such as an improved tensile strength, strain and toughness as a result of blending a peptide or polyamino acid having a &#x3b2;-sheet structure with a natural or artificially modified fibroin-derived protein; a method for producing such composite molding composition; and a method for improving the physical properties of a composite molding composition containing a fibroin-derived protein.

Composite molding composition including fibroin-like protein, and method for producing composite molding composition

Provided is a molded article of a composition comprising a polypeptide, wherein the polypeptide is at least one kind selected from the group consisting of natural spider silk protein and polypeptides derived from natural spider silk protein.

Molded article and method for producing molded article

A polypeptide solution of the present invention is a polypeptide solution in which a polypeptide derived from natural spider silk proteins is dissolved in a solvent. The solvent contains at least one selected from the following (i)-(iii): (i) DMSO; (ii) DMSO with an inorganic salt; and (iii) DMF with an inorganic salt. Further, in the present invention, an artificial polypeptide fiber is obtained by: using the polypeptide solution as a dope solution; and extruding the dope solution from a spinneret into a desolvation bath so as to eliminate the solvent from the dope solution and form a fiber to produce an undrawn yarn. Moreover, in the present invention, a polypeptide is purified by subjecting the polypeptide solution to heat treatment and thereafter removing an undissolved substance therefrom. Thus, the present invention provides the polypeptide solution whose solute has high solubility and solvent itself is low cost, and that allows dissolution at high temperatures and has high safety: a method for producing an artificial polypeptide fiber: and a method for purifying a polypeptide.

Polypeptide solution, artificial polypeptide fiber production method and polypeptide purification method using same

Provided is a method of determining a DNA tag which is a base sequence to be introduced into a genomic DNA sequence of an organism and an introduction site of the DNA tag into the genomic DNA sequence. The method includes: a step (S) of obtaining a protein coding sequence of the genomic DNA sequence; a step (S) of determining a region to be treated in the protein coding sequence; a step (S) of fragmenting the coding sequence in the region to be treated; a step (S) of subjecting the fragmented coding sequences to a silent mutation; a step (S) of determining sequences suitable as primers from polynucleotides including the fragmented coding sequences or complementary base sequences thereof; a step (S) of performing a homology search for the coding sequences thus determined; and a step (S) of determining the determined coding sequence corresponding to a minimum value of an NMS as the DNA tag, and determining the site of the fragmented coding sequence corresponding to the determined coding sequence in the protein coding sequence as the introduction site.

Method of making DNA tag

Growing community of inventors

Tsuruoka, Japan

Junichi Sugahara