Seikagaku Corporation

Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation)

Junichi Onaya

Satoshi Miyauchi

Yusuke Hori

Koji Yamamoto

Keiichi Yoshida

Hiroyuki Masuda

Takeshi Ishimaru

Hiroshi Fujita

Hideo Mochizuki

Akira Tawada

Yasuhiro Kurahashi

Masami Iida

Yuji Matsuzaki

Saburo Hara

Yousuke Yasuda

Mamoru Kyogashima

Yutaka Kariya

Shin-ichi Ishikawa

Shigeyuki Wakitani

Kaeko Kamei

Ryo Takano

Mikio Arai

Akikazu Takada

Shin-Ich Ishikawa

The inventions provides a method for immunologically determining a keratan sulfate level which method includes bringing an anti-keratan sulfate monoclonal antibody into contact with a biological sample, the anti-keratan sulfate monoclonal antibody exhibiting a relative reaction specificity between keratan sulfate-I and keratan sulfate-II represented by IC50of 0.4 to 5, to thereby provide a signal; and detecting keratan sulfate contained in the biological sample from the signal. On the basis of the method, the invention also provides a joint disease detection method and a method for assessing the effect of a remedy for a joint disease and a candidate substance therefor. Through these methods, a very small amount of keratan sulfate contained in a sample, can be determined. Particularly, these methods can determine, at high-sensitivity and high-specificity, the total keratan sulfate including keratan sulfate-I, which have been difficult to determine through a conventional technique. The methods also enables detect a joint disease and assess the effect of a remedy for a joint disease or a candidate substance therefor.

Method for assaying keratan sulfate, assay kit therefor and method for detecting joint disease by using the same

A medical composition for protuberance of epithelium, which comprises a solution comprising a polysaccharide or a medically acceptable salt thereof, wherein the solution has a viscosity of: (1) from 50 to 500 mPa&#xb7;s at a shear rate of from 7.7 to 10.0 s; (2) from 45 to 300 mPa&#xb7;s at a shear rate of from 19.2 to 20.0 s; and (3) from 40 to 200 mPa&#xb7;s at a shear rate of from 38.3 to 40.0 s, when measured using a rotational viscometer at 25&#xb0; C., and a syringe filled with the medical composition.

Medical composition for protuberance of epithelium

Method for producing oligosaccharide, and novel oligosaccharide and pharmaceutical composition containing the same

There is provided a glycosaminoglycan having a backbone structure comprising a repetitive disaccharide bearing a uronic acid residue and a glucosamine residue, and having sulfate groups, wherein substantially no sulfate group bound to the hydroxyl group at the 6-position of the glucosamine residue in the backbone structure is detected as determined by a chemical disaccharide analysis method in which the glycosaminoglycan is decomposed with nitrous acid, reacted with para-nitrophenylhydrazine and analyzed by high performance liquid chromatography, and the molar % of a uronic acid residue having a sulfate group at the 2-position is not less than 45% relative to total uronic acid residues, which is calculated from a disaccharide composition obtained by an enzymatic disaccharide analysis method in which the glycosaminoglycan is digested with glycosaminoglycan-degrading enzymes and analyzed by high performance liquid chromatography. The glycosaminoglycan can be used as an active ingredient of pharmaceuticals.

Glycosaminoglycan and drug compositions containing the same

Dermatan sulfate compositions and antithrombotic compositions containing

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Tokyo, Japan

Junichi Onaya