Fujifilm Corporation

Junichi Katada

Naoyuki Nishikawa

Takayoshi Oyamada

Hideyuki Karaki

Hiroyuki Chiku

Atsuhiko Wada

Hiroki Terada

Mikinaga Mori

Taisei Nishimi

Morihito Ikeda

Katsuya Inana

Tomonori Nishio

Akira Wakabayashi

Keiji Shigesada

Yoshihiro Aburaya

Dai Ujihara

Masayasu Konishi

Noriyuki Kasagi

Kenji Narahara

Tadahiro Matsuno

Tomoya Ohara

Natsuki Oyabu

Daisuke Ogata

Akihiko Onodera

Tomonori Takeshige

An immunochromatography including steps of mixing an antigen-containable specimen and modified magnetic particles, which are magnetic particles modified with a substance having a specific affinity to the antigen; collecting the magnetic particles using magnetism; dissociating the modified magnetic particles to obtain an antigen-concentrated solution by mixing the collected magnetic particles with a dissociation solution, an amount of which is smaller than the antigen-containable specimen; obtaining a neutralized antigen-concentrated solution; spreading gold particle composite bodies on an insoluble carrier having a reaction site at which a second binding substance has been immobilized, in a state where the gold particle composite bodies which are composite bodies of an antigen in the neutralized antigen-concentrated solution and modified gold particles which are gold particles modified with a first binding substance are formed; capturing the gold particle composite bodies at the reaction site; and silver-amplifying the gold particle composite body.

Immunochromatography

An object of the present invention is to provide an immunochromatographic kit and a method, which are capable of detectingwith high-sensitivity and specificity. According to the present invention, an immunochromatographic kit for detectingis provided, the kit including: a label substance modified with a first antibody against lipoarabinomannan; a porous carrier having a reaction site holding a second antibody against lipoarabinomannan; a compound containing silver; and a reducing agent reducing silver ions, in which at least one of the first antibody or the second antibody is a monoclonal antibody.

Immunochromatographic kit and method for detecting mycobacterium tuberculosis

A nozzle includes a mounting opening that is to be connected to an opening portion of a container, an outlet that jets liquid stored in the container, a side face that connects the mounting opening to the outlet, and a liquid storage portion that stores a part of the liquid stored in the container in the nozzle without jetting a part of the liquid from the outlet in a case where the liquid is to be jetted from the dispensing container, that is, in a case where the outlet faces downward in the direction of gravity.

Nozzle and dispensing container

It is an object of the present invention to provide an immunochromatography method capable of measuring a sample in a low concentration range and a high concentration range by setting the measurable range of the sample much wider than the conventional measurable range. The present invention provides an immunochromatography method, which comprises: spreading a test substance, a first labeling substance modified with a first binding substance that binds to the test substance, and a second labeling substance modified with the first binding substance that binds to the test substance, which are in a mixed state, on an insoluble carrier; and capturing the test substance, the first labeling substance, and/or the second labeling substance at a reaction site on the insoluble carrier having a substance having ability to bind to the first binding substance that binds to the test substance, so as to detect the test substance, wherein the first labeling substance is a labeling substance that causes an amplification reaction, and the second labeling substance is a labeling substance that does not substantially influence on the amplification reaction.

Immunochromatography method

Provided are a base plate and a method for thyroxine immunoassay, which are capable of decreasing the signal change rate caused by temperature change in a high-sensitivity immunoassay method for thyroxine. A base plate for thyroxine immunoassay which has a conjugate of thyroxine or a derivative thereof and albumin immobilized thereon, and in which the ratio of the molecule number of thyroxine or a derivative thereof to the molecule number of albumin in the immobilized conjugate, is disclosed.

Thyroxine immunoassay using fluorescent particles

It is an object of the present invention to provide a measurement kit for developing a first developing solution and a second developing solution from different directions to suppress background noise, and an immunochromatography kit. The present invention provides a measurement kit, which comprises a first developing member for supplying a first developing solution and a second developing member for supplying a second developing solution, wherein the developing direction of the first developing solution is allowed to intersect with the developing direction of the second developing solution, so that development is carried out by developing the first and second developing solutions in different developing directions, and a water absorbent portion is established on the downstream of each of the developing directions.

Measurement kit and an immunochromatography method

The present invention provides an immunochromatographic device, which contains the following (a) and (b): (a) a first device part holding a first insoluble carrier used for developing a complex formed with an analyte and a labeling substance comprising a metal labeled with a first binding substance that can bind to the analyte and capturing the analyte and the labeling substance at a reaction portion containing a second binding substance that can bind to the analyte, and (b) a second device part holding a second insoluble carrier used for developing a liquid and a third insoluble carrier used for absorbing a liquid, in such a way that the first insoluble carrier does not come into contact with the second insoluble carrier and the third insoluble carrier.

Immunochromatographic device

A device for assay can evenly develop solution, and performs highly accurate and sensitive measurement. A first device part () maintains a second insoluble carrier () and a third insoluble carrier () in such a manner that they overlap with each other at a detection portion () of a first insoluble carrier (). These three carriers (), () and () are housed not in contact with each other. A pressing unit () having a pressing surface () that is parallel to the detection portion () is provided on an inner surface of the second device part () facing the detection portion (). The pressing surface () is displaced by being pressed toward the detection portion (), and presses, from the upper side of the first insoluble carrier (), the second insoluble carrier () and the third insoluble carrier () onto the first insoluble carrier (). The first device part () and the second device part () are joined together.

Method and device for assay

The present invention is to provide an immunochromatography method which is capable of performing a detection with high-sensitivity or reduced false-positives by suppressing the occurrence of false-positive when a signal is amplified. An immunochromatography method includes in a state of where a complex of a test substance and a labeling substance containing a metal coupled with a first binding substance for the test substance is formed, developing the complex on an insoluble carrier in presence of a protease hydrolyzate of protein; capturing the test substance and the labeling substance at a detection site of the insoluble carrier containing a material which has a binding property to the first binding substance for the test substance or a second binding substance for the test substance; and detecting the test substance by amplifying the labeling substance captured.

Highly sensitive immunochromatography method and kit employing protein hydrolysates

A plurality of kinds of liquids, which are of at least three kinds, containing (a) a test body solution containing at least one kind of an analyte, and (b) at least two kinds of liquids selected from the group consisting of a reagent solution, an amplifying solution, and a detecting solution, are fed to a detection site containing a specific binding substance with respect to the analyte. A qualitative analysis or a quantitative analysis of the analyte contained in the test body solution is thereby performed. Directions of liquid feeding of all of the plurality of the kinds of the liquids vary from one another, and the plurality of the kinds of the liquids are caused to intersect with one another at the detection site.

Assay method

An immobilization method for immobilizing a physiologically active substance on a solid phase carrier, the method including: bringing the solid phase carrier into contact with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I); and carrying out a process of binding of the physiologically active substance to the acid anhydride functional group while maintaining the solid phase carrier after the contact at a temperature within the range of 0&#xb0; C. to 60&#xb0; C.; a physiologically active substance-immobilized carrier, and a carrier for immobilization are provided. Further, a carrier including a porous material treated with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I), a blocking agent that is immobilized to the porous material; and a method for producing it is provided. 

Method for immobilization, physiologically active substance-immobilized carrier, carrier for immobilization, carrier, and process for producing carrier

A chromatographic measurement apparatus for measuring a color development state of an insoluble carrier with a sample solution and a label solution developed thereon to test a test article, the insoluble carrier including a test detection area where a material that binds specifically to a test article is immobilized and a control detection area used for determining an end of measurement. The chromatographic measurement apparatus includes: a determining unit for carrying out determination of validity and determination of necessity of amplification of a test result of the test article; and an amplifying unit for amplifying the color development state according to the result of the determination of necessity of amplification by the determining unit. The amplification is carried out only when it is determined that amplification is necessary.

Chromatographic measurement apparatus

A object of the present invention is to provide an immunochromatography method that makes it possible to rapidly detect an ultratrace amount of an analyte that has been impossible to analyze by conventional immunochromatography methods. The present invention provides an immunochromatography method, which comprises developing an analyte and a labeling substance which is modified with a first binding substance against the analyte in a mixed state on a porous carrier and capturing the analyte and the label at a reaction site on the porous carrier having a second binding substance against the analyte or a substance capable of binding to the first binding substance against the analyte, so as to detect the analyte, wherein the labeling substance having an average particle size of 1 &#x3bc;m or more and 20 &#x3bc;m or less is detected.

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Ashigarakami-gun, Japan

Junichi Katada