Laboratory of Molecular Biophotonics

Junichi Ishida

Masatoshi Yamaguchi

Hitoshi Nohta

Kivoshi Zaitsu

Hiroko Iida

A method and a device for the analysis of 5-hydroxyindoles or catecholamines with high sensitivity. New chemiluminescence labeling agents, 6-aminomethylphthalhydrazide or 1,2-bis(phthalhydrazino)ethylenediamine, are reacted with 5-hydroxyindoles or catecholamines to form their stable derivatives. The derivatives emit strong luminescence in the presence of an oxidizing agent. In the chemiluminescence detection method, there is extremely low background noise and thus the method enables analysis with high sensitivity. The analysis of 5-hydroxyindoles or catecholamines in a sample is enabled by a method (1), in which each of the above-mentioned components is separated, reacted with said chemiluminescence labeling agent to form its derivative, and then chemiluminescent emitted by the reaction of the derivative with an oxidizing agent is detected, or by another method (2), in which the mixture of 5-hydroxyindoles or catecholamines in a sample is reacted with the chemiluminescence labeling agent to form its derivative mixture, subsequently each derivative is separated, and then chemiluminescent emitted by the reaction of the derivative with an oxidizing agent is detected.

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Fukuoka, Japan

Junichi Ishida