University of Pennsylvania

Agilent Technologies, Inc.

Junhyong Kim

James H Eberwine

Jai-Yoon Sul

Philip G Haydon

Fanyi Zeng

Ulo Langel

Chia-Wen Wu

Emelia Eiriksdottir

Daeyeon Lee

Yongwon Choi

Jinchun Wang

Mimi Healy

Jifen Li

Youtao Lu

Syung Hun Han

Tiina Peritz (nee Pajunen)

Tina Peritz

Jae-Hee Lee

Stephen Fisher

Provided is photoactivated selective release (or PHASR) of droplets from a microwell array enabled by a photoresponsive polymer layer integrated into the microfluidic device. This photoresponsive layer is placed in between a microwell array that traps a large number of droplets and a monolithic flow chamber that can be used for recovery. By using focused light, the photoresponsive layer can either be punctured or induced to create local heating to selectively release droplets. The type of photoacoustic dye and the physical properties of the photoresponsive layer can be engineered to induce either puncture of the membrane or pushing of droplets out of the microwells with low thermal impact on the droplets. This approach has broad application in the field of soft lithography-based microfluidic devices for various applications including photoresponsive valves as well as high throughput single cell sequencing.

Photoactivated selective release (PHASR) of droplets from microwell arrays

Provided herein are compositions and methods to assess the genomic landscape of fixed cells using light activated oligonucleotides that can be directed to the nucleus, mitochondria, or cytoplasm of fixed cells and that, upon activation, can be extended for in situ copying of nuclear single-stranded DNA (i.e., open chromatin), open mitochondrial DNA, and/or cytoplasmic RNA into barcoded complementary DNA. These methods also provide for gene specific 3D chromatin structural niche analysis.

Compositions and methods for genomic DNA and gene expression analysis in single cells

The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.

Transcriptome transfer produces cellular phenotype conversion

The present invention includes compositions, methods and kits for the real-time detection of transcription and translation in live cells, tissues and organisms. The present invention further provides method for the rapid sequencing of nucleic acids without using conventional sequencing techniques or reactions.

Compositions, methods and kits for real-time nucleic acid analysis in live cells

Methods of detecting transcription

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Narbeth, PA, United States of America

Junhyong Kim