William Marsh Rice University

Junghae Suh

Veronica Gough

Jonathan Silberg

Michelle Ho

Byunguk Kang

Momona Yamagami

Justin Judd

The present disclosure provide viral compositions and methods for modulating adeno-associated virus properties including transduction efficiency, virus capsid assembly, viral genome packaging, capsid stability and intracellular processing. Engineered adeno-associated viruses with modifications in the N-terminal region of the capsid proteins VP1 or VP2 are provided which have varying effects on viral properties including transduction efficiency. Corresponding nucleic acids and amino acids are provided.

Modification of n-terminal region of capsid proteins for enhanced properties of adeno-associated viruses

The present invention is a peptide lock that comprises at least one peptide that is genetically encoded into the Adeno-associated virus (AAV) capsid that block biologically active domains on the virus capsid surface. The peptide lock, can be processed by biological enzymes to restore biological behavior of the capsid-displayed domains, thus 'decoding the lock' or opening the lock. A method of forming the peptide lock comprises providing at least one peptide, providing an Adeno-associated virus capsid and genetically inserting the at least one peptide into the Adeno-associated virus capsid to block the biologically active domains on the virus capsid surface.

Encryption of adeno-associated viruses with enzymatically decoded peptide locks

Growing community of inventors

Houston, TX, United States of America

Junghae Suh