Konkuk University Industrial Cooperation Corp

Osong Medical Innovation Foundation, Korea University Research and Business Foundation

Intelligent Synthetic Biology Center

Biongene Co., Ltd.

Jung-Kul Lee

Tae-Su Kim

Jinglin Li

Ji-Hyun Park

Sun-Chang Kim

Youn-Woo Lee

Yun Chan Kang

Jing-Lin Li

Sigdel Sujan Chandra

Soo-Ryun Cheong

Sang-Young Kim

Ranjitha Ramakrishnan

Hyeon-Cheol Lee

Liaoyuan Zhang

Ramakrishnan Ranjitha

Hyung-Moo Jung

Chandra Sujan Sigdel

Saurabh Dhiman

Bong-Seoung Koo

In a method for producing acetoin, butanediol, or butanol from ethanol according to the present invention, a cell-free catalysis method was used by designing an artificial synthetic pathway so that proteins of NOX, EtDH, FLS, BDH, and DDH and variant proteins thereof exhibit cascade catalytic activity as enzymes. Compared to existing fermentation methods using microorganisms, the production method according to the present invention does not require cell growth and has a short synthetic pathway, a fast reaction rate, high yield and productivity, adjustment of targeted reaction conditions is convenient, and butanol may be effectively produced. Moreover, same may be reused numerous times by fixing the proteins to nano-particles, and are also effective for producing acetoin, butanediol, or butanol, thus being economical. Therefore, the production method may be usefully adopted in the relevant industries requiring acetoin, butanediol, or butanol.

FLS variant having increased activity

The present invention relates to improving the enzymatic activity ofO157:H7-derived sugar isomerase by molecular mechanics and site-directed mutagenesis. More particularly, the present invention relates to a sugar isomerase having an enzymatic activity improved by mutation, a nucleic acid molecule encoding the same, a vector comprising the nucleic acid molecule, a transformant comprising the vector, a mutant of the sugar isomerase, and a method for producing L-gulose using the improved sugar isomerase.

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This invention relates to a novel formaldehyde dehydrogenase expressed by a formaldehyde dehydrogenase gene and having independent reduction activity for formic acid, a method of preparing the formaldehyde dehydrogenase from a strain transformed with a recombinant expression vector including the gene, and a method of producing formaldehyde from formic acid through a reduction reaction of the formaldehyde dehydrogenase.

Formaldehyde dehydrogenase and method for preparing formaldehyde using same

Provided are a method of preparing biofuel, in which the method includes obtaining saccharificated products from lignocelluosic biomass in high saccharification yield, and a simultaneous pretreatment and saccharification (SPS), in which the method includes a first step of pulverizing biomass and immersing the pulverized biomass; and a second step of preparing saccharificated products by performing the simultaneous pretreatment and saccharification of the immersed biomass with the enzyme produced by inoculating a fungal consortium in a culture medium and culturing the fungal consortium. In addition, the second step further includes a detoxification of decreasing or removing toxic materials through laccase produced by culturing with a strain producing laccase in the second step.

Simultaneous pretreatment and saccharification of biomass using fungal consortium and method of preparing biofuel using the same

The present invention provides a newly isolated mutant culture ofBNG 2315. This culture is capable of producing teicoplanin more than 60 times productivity than those reported before (e.g.,nov. sp. ATCC 31121). The present invention also provides a fermentation process for the production of teicoplanin in an aerobic condition using the mutant strainBNG 2315 in a culture-medium comprising carbon sources, nitrogen sources and mineral salts.

Process for the production of teicoplanin

Growing community of inventors

Seoul, South Korea

Jung-Kul Lee