Chinese Academy of Agricultural Sciences

Chinese Academy of Sciences

Junbo Gou

Sanwen Huang

Qinggang Liao

Ran Du

Jianbin Yan

Chong Li

Haifei Liang

Xingyao Xiong

Provided are a taxadiene synthase TcTS2, an encoding nucleotide sequence and use thereof. The amino acid sequence of TcTS2 includes or consists of: (a) an amino acid sequence represented by SEQ ID NO: 1; or (b) a functional homologous sequence having at least 80% sequence similarity with the amino acid sequence represented by SEQ ID NO: 1; or (c) an amino acid sequence having TcTS2 activity with addition, deletion, or substitution of one or more amino acids in the amino acid sequence represented by SEQ ID NO: 1. TcTS2 and the nucleotide sequence encoding the TcTS2 provide new gene resources for improving the yield of taxol, and they may be used for modifying chassis hosts by plant genetic engineering and metabolic engineering strategies to produce taxol and the intermediates thereof etc., thereby having significant economic and social value.

Taxadiene synthase TcTS2, encoding nucleotide sequence and use thereof

Provided is primers, where the primers specifically bind to a polynucleotide and are used to amplify the polynucleotide; and the polynucleotide comprises or consists of the following sequence: 1) the nucleotide sequence represented by SEQ ID NO: 5; or 2) the complete complementary sequence of the sequence represented by SEQ ID NO: 5; or 3) a nucleotide sequence being identical to 300 bp or more contiguous nucleotides in the sequence of the above 1) or 2), where the nucleotide sequence being identical to 300 bp or more contiguous nucleotides is unique to a maleplant; and the sequences of the primers are respectively represented by SEQ ID NO: 1 and SEQ ID NO: 2.

Polynucleotide for identifying the sex of aplant and use thereof

Growing community of inventors

Shenzhen, China

Junbo Gou