Packard Bioscience Company

Perkinelmer Las, Inc.

Jun Yang

Todd J Stephan

David A Noblett

Mack J Schermer

Roger Dowd

A method and system are disclosed for automatically creating crosstalk-corrected data of a microarray utilizing calibration dye spots each of which comprises a single pure dye. A microarray scanner, such as a confocal laser microarray scanner, generates dye images, each of which contains at least one of the calibration dye spots for each of the output channels of the scanner. For each of the calibration dye spots, an output of each of the output channels is measured to obtain output measurements. A set of correction factors is computed from the output measurements to correct the data subsequently gathered from the microarray scanner. In other words, the correction factors are applied to quantitation or measurement data obtained from microarray images which contain spots having dyes of known or unknown excitation or emission spectra to obtain crosstalk-corrected data.

Method and system for automatically creating crosstalk-corrected data of a microarray

Method and system are disclosed for interactively developing at least one grid pattern, such as a microarray pattern, as well as an array set of such patterns, and a computer-readable storage medium having a program for executing the method given only a few user inputs through the use of a pointing device such as a mouse or trackball on a personal computer or workstation. Each array in the set typically has an identical number of rows and columns of points.

Method and system for interactively developing at least one grid pattern and computer-readable storage medium having a program for executing the method

Method and system for overlaying at least three microarray images to obtain a multicolor composite image, which is then displayed on a monitor of a computer system. The microarray images are taken from a microarray scanner of a DNA microarray and can be viewed simultaneously through the use of the image overlays where each image is represented by a different color. Each pixel of the composite image is generated by the OR operator applied to all corresponding pixels of the microarray images. Registration of the microarray images can be altered with a keyboard or mouse of the computer system.

Growing community of inventors

Santa Monica, CA, United States of America

Jun Yang