Bioright Worldwide Company Limited

Other

The Chinese University of Hong Kong

Geneharbor (Hk) Technologies Ltd.

The University of Hong Kong

Hong Kong Baptist University

Geneharbor (Hong Kong) Biotechnologies Limited

Geneharbor ( Hong Kong) Technologies, Ltd.

Jun Wang

Rongzhao Fu

Dong Shen

Caike Jin

Yau Lung Siu

Junming Chen

Paul Pui-Hay But

Shiu Ming Cheng

Yuk Sun Lui

Supyin Tsang

Hongkin Yap

Waikei Tsang

Zhaoxiang Bian

Chung Cheung

Liang Sun

Alfred K Wong

Qingjing Yang

Edmund Lam

Ngan Fai Karenda

Fai Ngor Karenda Ngan

Pang Chi Shaw

Shaw Pang Chui

Pui-Hay Paul But

Zihao Wang

Forrest C F Yau

Wai-Yan Ha

Pang-Chui Shaw

Guilin Wang

Zhangming Liu

Minghon Yau

Yaulung Philip Siu

Yuksun Lui

Guiwu Xiao

Lianquan Gu

Tianzuo Zhang

Yaulung Siu

Junmin Chen

Tin Cho Cheung

Yuen Wing Chow

Wing Keung Eric Poon

Chung Hong Cheung

Provided herein are compounds useful in the treatment of inflammatory diseases, pharmaceutical compositions comprising the same, and methods of use and preparation thereof. The compounds exhibit inhibitory effects on the expression and secretion of pro-inflammatory cytokines, such as IL-1β, IL-6 and TNF-α.

Flavan-3-ol analogs and anti-inflammatory activity

Provided herein are a device and a method for preparation of immobilized proteins, enzymes or cells on a carrier to achieve the industrial batch production of the immobilized proteins, enzymes or cells.

Device and a method for immobilization of proteins, enzymes or cells

Provided herein are penicillin expandase mutants, DNA coding the mutants, reagent kit containing the mutants and the application. The penicillin expandase mutants using SEQ ID NO.: 2 in the Sequence Listing as a reference sequence, have at least one amino acid mutation at residue positions corresponding to threonine at position 42 and glutamine at position 126, wherein, amino acid at position 42 is substituted by any other natural amino acid except threonine, amino acid at position 126 is substituted by any other natural amino acid except glutamine. The penicillin expandase mutants of present invention have increased its thermostability and catalytic activity; it is more suitable for commercial and industrial applications.

Penicillin expandase mutants, DNA coding the mutants, reagent kit containing the mutants and the application

This invention provides deacetoxycephalosporin C hydroxylase mutants, their encoding DNA sequences, the methods to utilize them and their application. The deacetoxycephalosporin C hydroxylase mutants are characterized by at least one amino acid mutation at residue position selected from Glycine at position 29, Alanine at position 40, Glycine at position 41, Arginine at position 182 and Threonine at position 272, based on the amino acid sequence shown in SEQ ID NO.:2 as reference sequence, and wherein the deacetoxycephalosporin C hydroxylase mutant has at least 10% increase in activity and at least 150% increase in thermostability compared to wild-type deacetoxycephalosporin C hydroxylase. The deacetoxycephalosporin C hydroxylase mutants in this invention have increased activity and thermostability, allowing them to be more commercially and industrially viable.

Deacetoxycephalosporin C hydroxylase mutants, DNA encoding the mutants, method for utilizing the mutants and application thereof

The present invention provides an immobilized reaction device and a method for carrying out reaction by utilizing the immobilization technology. The immobilized reaction device includes a columnar reactor with an inlet and an outlet. The reactor is provided with an interior cavity which is defined by a top portion and a bottom portion opposite to each other, and a side wall connecting the top portion and the bottom portion.

Immobilized reaction device and a method for carrying out a reaction by utilizing the immobilization technology

The invention discloses a carrier made from an organic foam having open pores for enzymes or cells immobilization and the methods for preparing immobilized enzymes or cells. The invention uses flocculation and crosslinking technology to immobilize enzyme protein or cells on the organic foam material having open pores. The resultant immobilized products have larger specific surface area, higher specific activity and can be made into various shapes.

Carriers for enzyme or cell immobilization and immobilization method using the carriers

This invention provides a series of recombinantglucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gin) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.

Isolated DNA encoding recombinant glucose isomerase

This invention provides use of a series of recombinantglucose isomerases with improved catalytic activity obtained by using recombinant techniques. These mutants comprise at least one amino acid variation at position 87, position 139, position 182, position 187, position 217, position 260, position 276, or position 299, and can be used in the conversion of hemicellulose to ethanol.

Method of making bioethanol by using glucose isomerase mutants

The present invention discloses a two-step enzyme method for preparing 7-aminocephalosporanic acid from cephalosporin C, wherein D-amino acid oxidase used is purified D-amino acid oxidase mutant of yeast, having a specific activity of 105% higher than that of parent D-amino acid oxidase. The method has no need of addition of hydrogen peroxide, &#x3b2;-lactamase inhibitor, catalase inhibitor, catalase and the like commonly used in the prior art. The productivity of the method can reach more than 93%. Thus, the method is simple, low in cost and high in productivity.

Two-step enzyme method for preparing 7-aminocephalosporanic acid

Methods of using isolated glucose isomerase

This invention describes a series of recombinantglucose isomerases having improved catalytic activity and thermostability. The recombinant glucose isomerases can be used for direct production of fructose syrup containing 55 wt % or higher concentration of fructose, or used for the production of fructose syrup containing less than 55 wt % fructose.

Glucose isomerase mutants

The invention discloses a series ofS-adenosylmethionine synthetase mutants with improved thermostability and high catalytic activity obtained by using gene mutation technique, characterized in that these mutants refer to an enzyme using Sequence 2 in the Sequence Listing as the reference sequence and contains at least one mutation at position 102, position 93, position 230, and position 357 and has a catalytic activity at least 70% higher than that of the wild-type S-adenosylmethionine synthetase using adenosine triphosphate (ATP) and methionine as substrates. These S-adenosylmethionine synthetase mutants can be used in the production of S-adenosylmethionine.

S-adenosylmethionine synthetase mutants, the DNAs encoding the same and uses of the mutants

This invention provides a series of recombinantglucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gln) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55.% [wt] or higher concentration of fructose.

Glucose isomerase mutants, DNA thereof and use thereof

An optical lithography method is disclosed that uses double exposure of a reusable template mask and a trim mask to fabricate regularly-placed rectangular contacts in standard cells of application-specific integrated circuits (ASICs). A first exposure of the reusable template mask with periodic patterns forms periodic dark lines on a wafer and a second exposure of an application-specific trim mask remove the unwanted part of the dark lines and the small cuts of the dark lines left form the rectangular regularly-placed contacts. All contacts are placed regularly in one direction while unrestrictedly in the perpendicular direction. The regular placement of patterns on the template mask enable more effective use of resolution enhancement technologies, which in turn allows a decrease in manufacturing cost and the minimum contact size and pitch. Since there is no extra application-specific mask needed comparing with the conventional lithography method for unrestrictedly-placed contacts, the extra cost is kept to the lowest. The method of the invention can be used in the fabrication of standard cells in application-specific integrated circuits (ASICs) to improve circuit performance and decrease circuit area and manufacturing cost.

Rectangular contact lithography for circuit performance improvement and manufacture cost reduction

The present invention relates to the o-pyridinequinone disubstituted derivatives, the composition containing the derivatives, the process for preparation of the derivatives and the use of the derivatives. The o-pyridinequinone disubstituted derivatives of the present invention have action of selectivity inhibiting COX-2 and they can be made into pharmaceuticals. The o-pyridinequinone disubstituted derivatives of the present invention have no acute toxicities and are a safe pharmaceutical.

O-pyridinequinone derivatives, the composition containing the derivatives, the process for preparation of the derivatives and the use of the derivatives

Growing community of inventors

Hong Kong, China

Jun Wang