Japan Tobacco Inc.

Jun Ueki

Shinji Morioka

Takeshi Yamamoto

Yoshimitsu Takakura

Yukoh Hiei

Yuji Ishida

Shozo Ohta

Yoshiki Kuraya

The present invention provides a method for-mediated gene transfer into a plant material, which comprises inoculating aninto the plant material in the presence of a powder. In the method of the present invention, the powder at least does not affect living tissues and has one or more properties selected from the group consisting of: being insoluble in water; having an affinity for living tissues; having adsorption properties; and having a surface polarity. The present invention also provides a method for producing a transformed plant, which comprises using the gene transfer method of the present invention.

Method for improving transformation efficiency using powder

A novel nucleic acid fragment which has an high activity to promote expression of a structural gene located downstream thereof, and a method for promoting expression of the structural gene located downstream thereof using the nucleic acid are disclosed. A nucleic acid fragment having the nucleotide sequence shown in SEQ ID NO:1 in the Sequence Listing, and nucleic acid fragments having the same sequence as shown in SEQ ID NO:1 except that one or more nucleotides are substituted or deleted, or one or more nucleotides are inserted therein or added thereto, which have activities to promote expression of a structural gene located downstream thereof were provided.

Nucleic acid fragment, recombinant vector containing the same and method of promoting the expression of structural genes by using the same

Nucleic acid fragments having activities to prominently promote expression of structural genes located downstream thereof are disclosed. The nucleic acid fragment according to the present invention is an isolated nucleic acid fragment having the nucleotide sequence shown in SEQ ID NO: 1 in Sequence Listing or an isolated nucleic acid fragment (excluding the nucleic acid having the nucleotide sequence shown in SEQ ID NO: 3 in Sequence Listing) having the same nucleotide sequence as shown in SEQ ID NO: 1 except that one or a plurality of nucleotides are substituted or deleted, or except that one or a plurality of nucleotides are inserted or added, which has an activity to promote expression of a structural gene located downstream of said nucleic acid fragment.

Nucleic acid fragments, recombinant vector containing the same and method for promoting the expression of structural gene by using the same

A novel DNA fragment capable of enhancing the expression level of a gene. The DNA fragment according to the present invention contains a nucleotide sequence from one of the nucleotides at around positions 1- to 152 to one of the nucleotides at around positions 259 to 374 in the sequence represented by SEQ ID NO:1 and is capable of enhancing the expression level of a gene.

DNA fragment elevating gene expression dose

A method for expressing a foreign gene, by which the foreign genes can be expressed more strongly than by the conventional methods, as well as a recombinant vector therefor, is disclosed. In the method for expressing a foreign gene according to the present invention, the foreign gene is inserted into a site downstream of a promoter and the foreign gene is expressed in a cell, wherein a plurality of intron-originated DNA fragments which are the same or different and are capable of promoting expression of foreign genes are inserted into one or more sites upstream of the foreign gene, and said foreign gene is expressed.

Growing community of inventors

Shizuoka, Japan

Jun Ueki