Takara Bio Inc.

Takara Shuzo Co., Inc.

Jun Tomono

Ikunoshin Kato

Hiroaki Sagawa

Takeshi Sakai

Yoshiko Nomura

Tatsuji Enoki

Harumi Ueno

Susumu Tsunasawa

Kiyozo Asada

Takashi Uemori

Nobuto Koyama

Eiji Kobayashi

Katsushige Ikai

Hiroyuki Mukai

Eiji Nishiyama

Takanari Tominaga

Yoshimi Sato

Junko Yamamoto

Kazue Miyake

Hua-Kang Wu

Michio Hagiya

Osamu Takeda

Akihiko Kita

Tetsuki Tanaka

Masayuni Kishimoto

Mariko Moriyama

Haruhisa Sawaragi

Yukiko Izu

Tetsuo Tanigawa

Masaru Miyagi

Production of efficiently producing agaro-oligosaccharide; a polypeptide having an agarase activity which is usable in, for example, efficiently extracting a substance such as a nucleic acid from an agarose gel; the amino acid sequence of the polypeptide; a gene encoding the polypeptide; a process for producing the polypeptide; a process for producing agaro-oligosaccharide; and a process for extracting a substance such as a nucleic acid from an agarose gel.

Agarase and gene thereof

A polypeptide having an &#x3b1;-agarase activity, a gene encoding the polypeptide, a method for producing the polypeptide by genetic engineering and a method for producing an agarooligosaccharide using the polypeptide.

&#x3b1;-agarase and process for producing the same

A gene encoding a polypeptide having an activity of digesting sulfated fucogalactan which is useful in sugar chain engineering reagents, analyzing the structures of sulfated fucose-containing polysaccharides and preparing degraded products of the polysaccharides; a genetic engineering process for producing the polypeptide; and the polypeptide obtained by the process.

Sulfated fucogalactan digesting enzyme gene

A vector having a region encoding a cold shock protein gene mRNA-origin 5'-nontranslated region, characterized in that the 5&#x2032;-nontranslated region has a mutation having been transferred therein so as to change the distance of the stem structure formed by the region.

Cold-induced expression vector

&#x3b1;-Agarase and process for producing the same

A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.

Method for amplifying nucleic acid sequence

Medicinal compositions

A polypeptide having an &agr;-agarase activity, a gene encoding the polypeptide, a method for producing the polypeptide by genetic engineering and a method for producing an agarooligosaccharide using the polypeptide.

&agr;-agarase and process for producing the same

Therapeutic agents

A method for performing site-directed mutagenesis characterized in that the method includes the step of carrying out PCR by the use of a double-stranded DNA vector having one or more amber codons, the vector resulting from insertion of a target DNA fragment for site-directed mutagenesis, and at least two kinds of selection primers; and a kit for site-directed mutagenesis for use in the above method, characterized in that the kit includes amber codon reversion primers. According to the present invention, there can be provided a method for performing site-directed mutagenesis and a kit, which is useful for genetic engineering or protein engineering, more simply and rapidly. By using the method and the kit of the present invention, it is possible to efficiently obtain a mutation-introduced gene at the desired position by simply transforming a host with a PCR product obtained by PCR.

Method for effecting site-directed mutagenesis

To provide an amino terminal protecting group-releasing enzyme characterized in that the enzyme possesses an activity for releasing a protecting group by acting on a peptide of which amino terminal is blocked by the protecting group, and exhibits the activity for two or more protecting groups, or a functional equivalent thereof; a DNA encoding the same; a method for producing the enzyme; a method for removing amino terminal protecting group including the step of subjecting to a reaction with the enzyme to release amino terminal protecting group; and a method for analyzing an amino acid sequence. The above enzyme is useful in the analysis of an amino acid sequence of peptides, particularly proteins and peptides, of which amino terminal is blocked by unknown protecting groups.

Amino-terminal deblocking enzyme

Growing community of inventors

Shiga, Japan

Jun Tomono