Kaneka Corporation

Jun Tomono

Shigehiko Miyamoto

Koji Takahashi

Sotaro Sano

Takaaki Jikihara

Hiroaki Inoue

Tatsumasa Mae

Hiromi Maeda

Shinichi Yokota

Kazuya Hamada

Tomohisa Kato

Hidehisa Kawahara

Yui Kawashima

Hiroyuki Hata

Hajime Hiratsuka

Yoshihide Koide

Naoki Arai

Akiko Kawai

Hitoshi Obata

Joichi Fukuoka

Hideaki Kegasa

An object of the invention is to provide a nucleic acid detection method which takes advantage of the high specificity of hybridization techniques, reduces the time length and the number of steps required for detection of PCR products, and allows for easy and highly accurate detection by visual observation without the need of special equipment; and a nucleic acid detection device or kit. The invention provides a method for detecting a target nucleic acid in a sample, which includes performing amplification of the target nucleic acid sequence to synthesize an amplification product having a partially double-stranded structure where a single-stranded region is added to each end of the target sequence, and hybridizing a nucleic acid sequence bound to a development medium and a nucleic acid sequence labeled with a labeling compound with the single-stranded regions of the amplification product to form a sandwich hybridization complex; and a detection device thereof.

Amplified nucleic acid detection method and detection device

Provided is a method for detecting small RNAs in a simple and highly accurate manner. Provided is a nucleic acid detection method including the following steps (a) to (c): (a) carrying out a reverse transcription reaction using a target RNA as a template and a reverse transcription primer having on its 5'-end side a sequence non-complementary to the target RNA to produce a reverse transcription product longer than the target RNA; (b) carrying out a nucleic acid amplification reaction using the reverse transcription product as a template and two primers to produce an amplified double-stranded DNA fragment having a single-stranded region at least at one end; and (c) hybridizing the single-stranded region of the amplified double-stranded DNA fragment to an oligonucleotide probe immobilized on a solid phase.

Micro RNA detection method using two primers to produce an amplified double stranded DNA fragment having a single stranded region at one end

A method and a device or kit for detecting a nucleic acid, which enable simple and precise visual detection of a nucleic acid amplified by an nucleic acid amplification method, without necessity of special devices are provided. The method for detecting a nucleic acid in a sample comprises: contacting a sample with a dye to react with each other; and observing a substance produced by the reaction with visible light, and evaluating the presence or absence of a nucleic acid by eye. The device or kit for detecting a nucleic acid in a sample comprises: a carrier that holds a dye which can bind to a nucleic acid; a path for passing a sample through the carrier; and an evaluation part for observing a substance produced by the reaction between the sample and the dye with visible light, and evaluating the presence or absence of a nucleic acid by eye.

Method for detecting nucleic acid, and device or kit

An object of the present invention is to provide methods for amplifying and detecting a nucleic acid that allow efficient hybridization, and devices and kits for use in the methods. The present invention includes amplifying a target nucleic acid into a double-stranded nucleic acid having a single-stranded region at each end, and detecting this nucleic acid. The present invention also provides detection devices and kits that make use of these methods.

Method for amplifying nucleic acid and method for detecting amplified nucleic acid

An object of the present invention is to provide a technique for preparing RNA ready for an enzymatic reaction more easily than conventional techniques. The present invention provides a reagent for RNA extraction from a biological sample which contains an alkali metal salt and a surfactant.

RNA preparation method

The present invention aims to solve problems in the analysis of amplified nucleic acids, i.e., cost, workability, and contamination and pollution of samples. In the present invention, a sample containing a nucleic acid and a reagent for detecting the nucleic acid are mixed in a closed system. In this regard, the nucleic acid is amplified in the same closed system, and then mixed with the detecting reagent without opening the system. In order to carry out this method, for example, a device that includes the detecting reagent shielded by a coating material or the like is used.

Nucleic acid detection method, and device and kit for use in same

Provided is a liquid food composition capable of semi-solidifying in the stomach, which is a one-pack type product containing a water-soluble dietary fiber preliminarily added thereto and in the form of a liquid that can be easily taken, and stably sustains the liquid nature thereof during distribution and storage. The liquid food composition, which is capable of semi-solidifying in an acidic region, comprises a water-soluble dietary fiber (a), a specific metal compound (b), a protein (c) and an emulsifier (d), and the particle size distribution of particles contained in said liquid food composition shows two or more peaks in a neutral region.

Treatment method using liquid food composition

The present invention provides a method for detecting a nucleic acid, by which a multi-stranded nucleic acid amplified by a nucleic acid amplification method is detected easily and with a high degree of accuracy without the need for specialized equipment, and also provides a nucleic acid detection device. Provided are a method for detecting a nucleic acid under visible light via a color reaction produced by contact between a chromogenic leuco dye and the multi-stranded nucleic acid, as well as a nucleic acid detection device using this method.

Method and device for detecting nucleic acid, and kit

In the present invention, an amplified DNA fragment having a first substance binding site to which a first substance is specifically bindable is prepared, which amplified DNA fragment amplified by a nucleic acid amplification method. The amplified DNA fragment is concentrated by binding the amplified DNA fragment to the first substance. The concentration makes it possible to detect the DNA highly sensitively. Therefore, with the arrangement, it is possible to detect the amplified DNA fragment amplified by the nucleic acid amplification method, easily and highly accurately without requiring any special device.

Nucleic acid detection method and nucleic acid detection kit

Combined micro tube and cap

Liquid food composition

The objective to be solved by the present invention is to provide an ice-crystallization inhibitor which has a practicable and excellent ice-crystallization inhibiting property and which can be efficiently and stably produced in a safe process suitable for food production. Also, the objective of the present invention is to provide an antibody which specifically reacts with the ice-crystallization inhibitor, and a composition, a food, a biological sample protectant and a cosmetic which contain the ice-crystallization inhibitor. Furthermore, the objective of the present invention is to provide a polysaccharide which is derived from a basidiomycete and which is used for inhibiting ice-crystallization of a liquid containing water, and a method for inhibiting ice-crystallization of a liquid containing water. The ice-crystallization inhibitor according to the present invention is characterized in being a polysaccharide derived from a basidiomycete.

Growing community of inventors

Takasago, Japan

Jun Tomono