Ajinomoto Co., Ltd.

Jun Takakura

Hiroyuki Nozaki

Isao Abe

Shunichi Suzuki

Hideyuki Suzuki

Rie Takeshita

Keiko Danjou

Hidemi Nagata

A method efficiently produces a compound containing an N-acyl-amino group by an enzymatic method. Specifically, a method of producing a compound containing an N-acyl-amino group includes producing the compound containing an N-acyl-amino group by reacting a compound containing an amino group with a compound containing a carboxyl group in the presence of an enzyme having an ability to bond a carboxyl group and an amino group in an ATP dependent manner to form an amide bond.

Method of producing N-acyl-amino group-containing compound

The present invention provides a method for producing N&#x3b5;-dodecanoyl-L-lysine using an enzyme having properties suitable for the industrial production of N&#x3b5;-dodecanoyl-L-lysine. More specifically, the present invention provides a method for producing N&#x3b5;-acyl-L-lysine including reacting a carboxylic acid or a salt thereof and L-lysine or a salt thereof in the presence of a protein having an amino acid sequence of SEQ ID NO: 1; a protein having an amino acid sequence in which one or several amino acid residues are inserted, added, deleted, or substituted in the amino acid sequence of SEQ ID NO: 1 and having N&#x3b5;-acyl-L-lysine-specific aminoacylase activity; and a protein which has 90% or higher homology with the amino acid sequence of SEQ ID NO: 1 and having N&#x3b5;-acyl-L-lysine-specific aminoacylase activity.

Method for producing N&#x3b5;-acyl-L-lysine

A method for producing &#x3b3;-Glu-Val-Gly comprising the step of reacting Val-Gly with a &#x3b3;-glutamyl group donor in the presence of a &#x3b3;-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate &#x3b3;-Glu-Val-Gly, wherein the &#x3b3;-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.

Method for producing gamma-glutamylvalylglycine or a salt thereof

Method for producing &#x3b3;-glutamylvalylglycine or a salt thereof

Growing community of inventors

Kawasaki, Japan

Jun Takakura