Green Cross Holdings Corporation

Jun Sic Kim

Ju Ho Lee

Hyo Jin Kim

Ji Yoon Park

Ki-Yong Kim

Yong Gyu Kang

Jae Woon Son

Yong Won Shin

Gun Sul Lee

Ki Hwan Son

Yong Kang

A method for purifying fibrinogen includes steps of: (a) precipitating fibrinogen of a fibrinogen-containing solution by adding glycine to the solution for a concentration of glycine to be 1.5 to 2.5M, and then removing a supernatant and recovering a precipitate (1glycine precipitation); (b) dissolving the precipitate of 1glycine precipitation of step (a) in a dissolution buffer to obtain a solution, precipitating the solution by adding glycine thereto for a concentration of glycine to be 0.2 to 1.2M, and recovering a supernatant (2glycine precipitation); (c) precipitating the supernatant of step (b) by adding glycine thereto for a concentration of glycine to be 1.5 to 2.5M, and recovering a precipitate (3glycine precipitation); and (d) dissolving the precipitate of step (c) in a dissolution buffer to obtain a solution, and subjecting the solution to nanofiltration using a nanofilter.

Method for purifying fibrinogen

A method for preparing a highly concentrated fibrinogen solution includes adding amino acid or amino acid derivatives, and/or salts to a lowly concentrated fibrinogen solution, followed by a ultra-filtration concentration. Factor XIII can be added either before or after the ultra-filtration to give a fibrin sealant componentcontaining the highly concentrated fibrinogen solution. The fibrin sealant componentcould be preserved for a long time at room temperature and be used without a reconstitution. Fibrin sealant componentis a solution containing thrombin and calcium. A fibrin sealant product may be provided in a vial type in which the fibrin sealant componentsandare each filled in separate vials or in a re-filled syringe type wherein the fibrin sealant componentsandare each filled in separate syringes connected with each other to be instantly used.

Method for preparing highly concentrated fibrinogen solution and method for preparing fibrin sealant by using thereof

Growing community of inventors

Yongin-si, South Korea

Jun Sic Kim