Ajinomoto Co., Ltd.

Amano Pharmaceutical Co., Ltd.

The Japanese Research & Development Association for Bioreactor System in

Jun Kurashige

Shotaro Yamaguchi

Tamio Mase

Narihide Matsuzaki

Tsuyoshi Nakamatsu

Naoki Usui

Naoto Kato

A process for modifying fats and oils which comprises applying partial glyceride lipase to fats and oils containing partial glycerides and fatty acids to synthesize triglycerides from the partial glycerides by esterification. The lipase is preferably an immobilized lipase and this process is preferably carried out under a water concentration of 1500 ppm or less. By using partial glyceride lipase according to the present invention, there can be obtained modified fats and oils having a low content of diglycerides without changing triglyceride composition.

Process for modifying fats and oils

Lipase is absorbed on a carrier to provide an immobilized lipase preparation containing 0.5 to 8% water by a process that does not require drying. The process is carried out by providing a solution or dispersion of lipase in a liquid solvent, mixing the solution or dispersion of lipase with a carrier to provide a mixture containing 0.5 to 8% water and kneading the mixture to produce the immobilized lipase preparation. The solution or dispersion of lipase may contain an enzyme activity enhancing agent such as a fatty acid or its derivative such as lecithin. By not drying, an immobilized lipase preparation having higher activity is obtained.

Preparation of an immobilized lipase having a low water content without

The present invention relates to a method for reforming fats and oils which comprises treating the fats and oils containing partial glycerides with two or more kinds of lipases which are different in a fatty acid specificity and/or a position specificity in the presence of a small amount of water to obtain fats and oils containing partial glycerides in a low content in a high yield. According to the invention, a reaction rate and a yield are improved.

Method for reforming fats and oils with enzymes

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Kawasaki, Japan

Jun Kurashige