Lg Chem, Ltd

Korea Advanced Institute of Science and Technology

Bionano Health Guard Research Center

Jun Ki Ahn

Hyun Gyu Park

Hang Beum Shin

Yong Min Ju

Hyo Yong Kim

Hun Park

Seung Jun Oh

Yong Ju

A biosensor with improved detection sensitivity of a target substance, a method for preparing a biosensor with improved detection sensitivity, and a method for detecting a target substance using the biosensor.

Biosensor and preparation method thereof

The present invention relates to a detection method for detecting a target RNA contained in a sample with high sensitivity by using nicking/extension chain reaction system-based isothermal nucleic acid amplification (NESBA) that uses activity of a cleavage enzyme and a DNA polymerase. The NESBA of the present invention is a new concept isothermal target RNA detection method that realizes higher amplification efficiency than the existing NASBA technology and is deemed to be utilizable as a new concept diagnosis technology that can replace conventional target RNA detection technologies.

Method for detecting target RNA by utilizing nicking/extension chain reaction system-based isothermal nucleic acid amplification

Growing community of inventors

Daejeon, South Korea

Jun Ki Ahn