Shimadzu Corporation

Jun-ichi Furukawa

Takashi Nishikaze

Hisatoshi Hanamatsu

Ikuko Yokota

A method for preparing an analysis sample from a sample containing a glycan, includes: performing esterification reaction that subjects at least a part of a sialic acid included in the glycan to esterification other than lactonization; and performing amidation reaction that converts an esterified form of a sialic acid modified through the esterification into an amidated form through contacting the sample with an amidation reaction solution containing at least one compound selected from the group consisting of ammonia, amines, hydrazine, hydrazine derivatives, and hydroxyamine, and salts thereof to be reacted with the sialic acid modified through the esterification.

Method for preparing analysis sample, analysis method, and kit for preparing analysis sample

A method for preparing a sample comprising a glycan includes: performing a lactonization reaction in which at least a part of sialic acids included in the glycan is lactonized; and performing an amidation reaction in which lactones of lactonized sialic acids are amidated through addition of an amidation reaction solution to the sample, the amidation reaction solution comprising at least one selected from the group consisting of ammonia, an amine, and a salt thereof that is reacted with the lactonized sialic acids.

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Sapporo, Japan

Jun-ichi Furukawa