Korea Advanced Institute of Science and Technology

Korea Atomic Energy Research Institute

Intelligent Synthetic Biology Center

Scarab Genomics, LLC

Jun Hyoung Lee

Choong Hoon Lee

Sun-Chang Kim

Tae Wan Kim

Frederick R Blattner

Young In Kim

Ju Young Lee

Bong Hyun Sung

Keung Koo Kim

Sun Chang Kim

Ju Hyeon Yoon

Young Min Bae

Soo Jai Shin

Cheon Tae Park

Joo Hyung Moon

Won Jae Lee

Byung-Kwan Cho

Han Ok Kang

Ki Jung Lim

Seung Yeop Yoo

Byung Jo Yu

Keun Bae Park

Myung Keun Park

Sang Hee Lee

Donghui Choe

Kyung Seok Yang

Kui Hyeon Kang

Provided is a method of producing a novel microorganism having a minimal genome. In particular, provided are a method of producing a novel microorganism having a smaller genome size than a wild-type, the method including the step of culturing the microorganism in a medium containing LB broth with stepwise decreasing concentrations of the LB broth; and a novel microorganism having a smaller genome size than a wild-type, produced by the above method. Despite the small genome size, the novel microorganism of the present disclosure may have a high growth rate, pyruvate production capacity, and protein translation efficiency thereby being usefully applied to industry such as production of recombinant proteins or pyruvate.

Microorganism having minimal genome and method of producing the same

The present disclosure may disclose a multi stage safety injection device and a passive safety injection system having the same, including a safety injection tank formed to contain coolant to be injected into a reactor vessel by a gravitational head of water when an accident occurs in which the pressure or water level of the reactor vessel is decreased, a pressure balance line connected to the reactor vessel and safety injection tank to form a pressure balance state between the reactor vessel and the safety injection tank, a safety injection line connected to a lower end portion of the safety injection tank and the reactor vessel to inject coolant to the reactor vessel in a pressure balance state between the reactor vessel and the safety injection tank, and a flow control line extended from the safety injection line to an inner portion of the safety injection tank, and provided with safety injection ports into which coolant is injected at predetermined heights, respectively, to reduce the flow rate of coolant injected into the reactor vessel step by step according to the water level reduction of the safety injection tank, in order to inject coolant to the reactor vessel at multi stages.

Multi stage safety injection device and passive safety injection system having the same

Disclosed are an amphipathic peptide-lipase conjugate with enhanced lipase activity, a polynucleotide coding for the conjugate, an expression vector carrying the polynucleotide, a transformant anchoring the expression vector therein, a method for preparing the conjugate, a lipolysis method using the conjugate, and a method for producing biodiesel using the lipase.

Amphipathic peptide-lipase conjugate having advanced lipase activity and use thereof

The present invention relates generally to compositions and methods useful for, inter alia, production of commercial biologic products such as amino acids. More specifically, the present invention relates to genetically modified strains of microorganisms and the use thereof for the production of commercial products. The present invention also provides, inter alia, novel isolated DNA, nucleic acid, vectors and reduced genome bacteria.

Compositions and methods for amino acid biosynthesis

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Daejeon, South Korea

Jun Hyoung Lee