Kao Corporation

Jun Hitomi

Mikio Takaiwa

Mitsuyoshi Okuda

Katsuhisa Saeki

Masafumi Nomura

Hiromi Kubota

Shitsuw Shikata

Yasushi Kageyama

Susumu Ito

Kazuaki Igarashi

Tohru Kobayashi

Shuji Kawai

Shigehito Adachi

Yoshihiro Hakamada

Norihiko Higaki

Tadashi Yoshimatsu

Yoko Watanabe

Eiichi Hoshino

Kazuo Mori

Akira Suzuki

Shuichi Takizawa

Shuuichi Takizawa

It is intended to provide a process for conveniently producing phosphorylase at a high purity using a phosphorylase-producing microorganism. Namely, a process for producing phosphorylase characterized by culturing a phosphorylase-producing microorganism in a medium containing phosphoric acid or its salt at a concentration of 50 mM or above and collecting the phosphorylase thus produced in the medium.

Process for producing phosphorylase

The present invention provides an M-1-P-producing enzyme which employs, as starting materials, an oligosaccharide or polysaccharide having a glucose polymerization degree of 5 or more and containing an &#x3b1;-1,4-glycosidic bond such as maltooligosaccharide, dextrin, or starch, and which enables production of a large amount of M-1-P. The M-1-P-producing enzyme can produce M-1-P from a phosphoric acid or a salt thereof and an oligosaccharide or polysaccharide having a glucose polymerization degree of 5 or more and containing an &#x3b1;-1,4-glycosidic bond.

Maltose-1-phosphate-producing enzyme

An alkaline protease having the following properties; a gene encoding the same; a microorganism producing the same; and washing compositions containing the same; (i) acting over a broad pH value range of 4 to 13 and achieving, at pH 6 to 12, 80% or more the activity at the optimum pH value; (ii) when treated at 40&#xb0; C. for 30 minutes, being stable over a pH value range of 6 to 11; (iii) having an isoelectric point of about 8.9 to 9.1; and (iv) having casein digesting activity that is not inhibited by oleic acid. The alkaline protease of the present invention is highly stable to various surface active agents and fatty acids, and exhibits high stability to oxidizing agents, and is therefore useful as an enzyme to be used in detergents for automatic dishwashers and laundry detergents, both containing bleaching components.

Alkaline protease

An alkaline protease having the following properties; a gene encoding the same; a microorganism producing the same; and washing compositions containing the same; (i) acting over a broad pH value range of 4 to 13 and achieving, at pH 6 to 12, 80% or more the activity at the optimum pH value; (ii) when treated at 40&deg; C. for 30 minutes, being stable over a pH value range of 6 to 11; (iii) having an isoelectric point of about 8.9 to 9.1; and (iv) having casein digesting activity that is not inhibited by oleic acid. The alkaline protease of the present invention is highly stable to various surface active agents and fatty acids, and exhibits high stability to oxidizing agents, and is therefore useful as an enzyme to be used in detergents for automatic dishwashers and laundry detergents, both containing bleaching components.

The present invention relates to a novel enzyme called alkaline proteinase K-16 that is characterized as having an optimum pH in an alkaline range, and stability in the presence of various surface active agents, such as sodium linear alkylbenzene sulfonate, sodium polyoxyethylene alkyl sulfate, sodium dodecyl sulfate, sodium .alpha.-olefin sulfonate, sodium alkyl sulfonate, and .alpha.-sulfo-fatty acid ester. The present invention further provides for the microorganism Bacillus sp. Ferm BP-3376, which is capable of producing the novel alkaline proteinase, and a process for producing the novel alkaline proteinase. Alkaline-proteinase K-16 of the present invention exhibits an excellent action against insoluble proteins and maintains sufficient activity over a wide temperature range and in the presence of surface active agents thus providing a use as an enzyme for detergents.

Bacillus SP. ferm BP-3376 and a method for its use to produce an

A detergent compositions comprising an alkaline protease, having an optimum pH range on the alkaline side, having an activity of hydrolyzing human horny keratin fiber of 40 KFU/APU or more, possessing a superior activity toward insoluble proteins, and exhibiting a sufficient activity in a wide temperature range, exhibit excellent detergency toward protein soils.

Detergent composition

The present invention relates to a novel enzyme called alkaline proteinase K-16 that is characterized as having an optimum pH in an alkaline range, and stability in the presence of various surface active agents, such as sodium linear alkylbenzene sulfonate, sodium polyoxyethylene alkyl sulfate, sodium dodecyl sulfate, sodium .alpha.-olefin sulfonate, sodium alkyl sulfonate, and .alpha.-sulfo-fatty acid ester. The present invention further provides for a microorganism producing the novel alkaline proteinase, and a process for producing the novel alkaline proteinase. Alkaline proteinase K-16 of the present invention exhibits an excellent action against insoluble proteins and maintains sufficient activity over a wide temperature range and in the presence of surface active agents thus providing a use as an enzyme for detergents.

Alkaline proteinase isolated from bacillus sp.

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Tochigi, Japan

Jun Hitomi