Gene Bridges Gmbh

Jun Fu

Adrian Francis Stewart

Youming Zhang

Wenli Zhang

Anja Ehrhardt

Marcello Maresca

Eric Ehrke-Schulz

Rolf Müller

Axel Steffen Erler

Xiaoying Bian

Philipp Martin Seibert

Francis Stewart

A method for performing homologous recombination between at least a first nucleic acid molecule and a second nucleic acid molecule which share at least one region of sequence homology. A method for improving the efficiency of homologous recombination.

Direct cloning

The invention relates to adenoviral vectors, cells for use in generating adenoviral vectors, methods for generating adenoviral vectors, and therapeutic uses of adenoviral vectors in gene therapy, tumour therapy and as vaccines.

Adenoviral vectors

This invention is related to bacterial engineering and the heterologous expression of useful compounds. In particular, the invention relates to a heterologous host that has been engineered for expression of a gene which is capable of polyketide or non-ribosomal peptide synthesis. Methods of treating cancer are also disclosed.

Heterologous hosts

The invention provides a method for inserting a single stranded replacement nucleic acid into a target nucleic acid, the method comprising the steps of: a) generating a single stranded replacement nucleic acid from a double stranded nucleic acid, wherein the double stranded nucleic acid is adapted at one or both of its 5' ends such that preferential degradation of one strand and/or strand separation generates the single stranded replacement nucleic acid, wherein the single stranded replacement nucleic acid comprises a 5&#x2032; region that is identical to sequence on the target nucleic acid, a 3&#x2032; region that is identical to sequence on the target nucleic acid and optionally a replacement region between the 5&#x2032; and 3&#x2032; regions that is not identical to sequence on the target nucleic acid, b) exposing the target nucleic acid to the single stranded replacement nucleic acid under conditions suitable for recombination to occur between the single stranded replacement nucleic acid and the target nucleic acid, and c) selecting a target nucleic acid whose sequence has been altered by inclusion of said single stranded replacement nucleic acid. Other methods for modifying target nucleic acids are also provided.

Method of nucleic acid recombination

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Dresden, Germany

Jun Fu